9°N™ Reverse Gyrase
Product Information
9°N™ Reverse Gyrase is a thermostable Type IA topoisomerase that introduces positive supercoils into double-stranded closed-circular DNA in the presence of ATP (1).
Product Source
An E. coli strain that carries a cloned gene from a thermophilic marine archaea Thermococcus sp. (strain 9°N) (2) with a C-terminal His-tag.
This product is related to the following categories:
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| M0200S | 9°N™ Reverse Gyrase | M0200SVIAL | -20 | 1 x 0.05 ml | 5,000 units/ml |
| Gyrase Reaction Buffer | B7636SVIAL | -20 | 1 x 1 ml | 5 X |
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to supercoil 0.5 µg of relaxed pUC19 plasmid in 15 minutes at 80°C, in a total reaction volume of 40 µl.
Reaction Conditions
Incubate at 80°C
Heat Inactivation
Advantages and Features
Features
- Introduces positive supercoiling in the presence of ATP
- Enhanced reaction buffer for improved activity
- Thermostable for high temperature workflow applications
Related Products
Companion Products
Product Notes
- The reaction requires ATP and Mg2+ (3, 4). The supplied Gyrase Reaction Buffer incorporates these essential components within an optimized formulation, thereby facilitating enhanced gyrase activity.
- In the absence of ATP, the enzyme will nick circular DNA (5).
References
Protocols, Manuals & Usage
Protocols for this product
FAQs & Troubleshooting
FAQs
Citations & Technical Literature
Citations
Product Citation Tool
Product Citation Tool
Bioz-powered product citation information for M0200.
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner.
