Nucleic Acid Purification
Product Overview
Workflows for detecting, analyzing, amplifying or manipulating DNA and RNA often require extraction and purification from a biological sample, such as blood, tissue and cultured cells, and/or enzymatic reactions. This process can include cleanup, which can concentrate nucleic acid from reactions or agarose gel.
Monarch® nucleic acid purification kits provide exceptional value with uncompromising quality
Monarch nucleic acid purification kits provide fast and reliable isolation and purification of high-quality DNA and RNA from a variety of sample types using best-in-class silica column technology and a novel glass bead-based approach for HMW gDNA extraction. DNA and RNA purified with Monarch Kits is highly pure and suitable for use in a wide variety of downstream applications including sequencing, cloning, PCR and other enzymatic manipulations. Monarch kits are all designed for sustainability, using less plastic than leading suppliers and are packaged in responsibly sourced, recyclable material – all while being competitively priced and providing more value for your research without compromising quality. For convenience and flexibility, Monarch kit columns and components are also available separately.
Select a product category to learn more
Plasmid Purification
PCR & DNA Cleanup
Total RNA Extraction & Isolation
Genomic DNA Extraction & Purification
RNA Cleanup
DNA Gel Extraction
HMW DNA Extraction
Magnetic Bead-Based Nucleic Acid Purification
Nucleic Acid Purification
Nucleic Acid Purification includes these subcategories:
Plasmid Purification
PCR & DNA Cleanup
DNA Gel Extraction
Genomic DNA Extraction & Purification
High Molecular Weight DNA Extraction
Total RNA Extraction & Purification
RNA Cleanup
Magnetic Bead-Based Nucleic Acid Purification
FAQs for Nucleic Acid Purification
- Can I use water to elute the DNA when using the Monarch Kits?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- Are plasmids recovered using the Monarch® Plasmid Miniprep Kit endotoxin free?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- What is the maximum binding capacity of the Monarch® Spin gDNA Extraction Kit columns?
- What is the minimum elution volume that can be used with the Monarch® Spin gDNA Extraction Kit columns?
- Can I use the Monarch® Spin gDNA Extraction Kit for gDNA cleanup?
- Can I use theMonarch® Spin gDNA Extraction Kit to clean up gDNA that was extracted with phenol/chloroform?
- Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
- Is Monarch-purified gDNA suitable for long-range PCR?
- What is the minimal input amount that can be used with the Monarch® Spin gDNA Extraction Kit?
- Can I use Monarch RNase A for applications/workflows other than gDNA purification?
Protocols for Nucleic Acid Purification
- Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
- Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Quick Protocol for Total RNA Extraction (NEB #T2010)
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Cultured Mammalian Cells
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - 100 ul Blood
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Tissue
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Buccal Swabs
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Gram-negative Bacteria
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Gram-positive Bacteria andArchaea
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Saliva
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Yeast
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Preparation - Genomic DNA Cleanup
- Quick Protocol for Extraction and Purification of Genomic DNA
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
- Protocol for Monarch Genomic DNA Extraction (NEB #T3010) Part 1: Sample Lysis - Up to 500ul Blood using Monarch RBC Lysis Buffer (NEB #T3051)
- Monarch® Spin RNA Cleanup Kit Protocol
- Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® Spin RNA Cleanup Kits
- Separation of Large and Small RNA into Fractions using the Monarch® Spin RNA Cleanup Kits
- Extraction of RNA from Agarose Gels using the Monarch® Spin RNA Cleanup Kits
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
Application Notes for Nucleic Acid Purification
- Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit
- A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers
- A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing
- Facilitating Purification and Detection of Viral Nucleic Acids from Milk
- A Sample to Results Workflow for Influenza A Sequencing
Tools & Resources
Feature Articles
Five Steps to a Greener Lab: A roadmap to environmental action
The laboratory is a place of inspiration, curiosity, ingenuity, and often altruism. Researchers are, by and large, a thoughtful and deeply invested group, but the environmental and ecological consequences of life science research are not typically at the forefront of researchers’ minds when planning their investigations.
Brochures
Monarch DNA Purification Brochure
Monarch RNA Purification Brochure
Troubleshooting Guides
Troubleshooting Guide for DNA Cleanup and Plasmid Purification
Troubleshooting Guide for RNA Cleanup
Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)
Troubleshooting Guide for Total RNA Extraction & Purification
Usage Guidelines
Six Tips for a Perfect Gel Extraction
Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch® Spin gDNA Extraction Kit
Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
Choosing Input Amounts for the Monarch Spin gDNA Extraction Kit
Dos and Don'ts of Plasmid Minipreps
Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
Guidelines for Working with RNA During RNA Cleanup
Tips for the Monarch PCR & DNA Cleanup Kit
Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids
Guidelines for Handling Tissue Samples when using the Monarch Spin gDNA Extraction Kit
Guidelines for RNA Quantitation
General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
Posters
A Novel Method for High Molecular Weight (HMW) DNA Extraction
Detection of Tumor-specific Mutations in Plasma Utilizing an Optimized Cell-free DNA Extraction Workflow
Optimization of cfRNA detection via sequencing: Addressing workflow challenges
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New Products
Monarch® Mag Cell-free RNA (cfRNA) Extraction Kit
Exo-CIP™ Rapid PCR Cleanup Mix
Featured Products
Monarch® Spin Plasmid Miniprep Kit
Monarch® Spin DNA Gel Extraction Kit
Monarch® Spin PCR & DNA Cleanup Kit (5 μg)
Monarch® Spin RNA Isolation Kit (Mini)
Monarch® Spin gDNA Extraction Kit
Featured Technical Notes
A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers
Featured Technical Resources
Choosing Sample Input Types for RNA Purification and Average Yields Expected
Tips for the Monarch PCR & DNA Cleanup Kit
Explore Monarch
Try a Monarch Sample
Experience Monarch sample preparation.
Learn about our sustainability efforts
Discover our sustainability initiatives.
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Nucleic Acid Purification
Nucleic Acid Purification Products
Cryogenic Cold Storage Box
Exo-CIP™ Rapid PCR Cleanup Kit
Exo-CIP™ Rapid PCR Cleanup Mix
Monarch® 2 ml Tubes
Monarch® Bead Retainers
Monarch® Buffer B2
Monarch® Buffer B3
Monarch® Buffer BX
Monarch® Buffer BY
Monarch® Buffer BZ
Monarch® Buffer WX
Monarch® Buffer WZ
Monarch® Collection Tubes II
Monarch® DNA Wash Buffer
Monarch® DNase I, Lyophilized
Monarch® gDNA Binding Buffer
Monarch® gDNA Blood Lysis Buffer
Monarch® gDNA Cell Lysis Buffer
Monarch® gDNA Elution Buffer
Monarch® gDNA Elution Buffer II
Monarch® gDNA Nuclei Prep & Lysis Buffer Pack
Monarch® gDNA Tissue Lysis Buffer
Monarch® gDNA Wash Buffer
Monarch® HMW DNA Extraction Kit for Cells & Blood
Monarch® HMW DNA Extraction Kit for Tissue
Monarch® HMW gDNA Tissue Lysis Buffer
Monarch® Mag Beads M2
Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit
Monarch® Mag Cell-free RNA (cfRNA) Extraction Kit
Monarch® Mag PCR & DNA Cleanup Kit (5 μg)
Monarch® Mag Viral DNA/RNA Extraction Kit
Monarch® Microfuge Tube EcoRack
Monarch® Pestle Set
Monarch® Plasmid Miniprep Columns
Monarch® Precipitation Enhancer
Monarch® Protein Separation Solution
Monarch® RBC Lysis Buffer
Monarch® RNase A
Monarch® Spin Collection Tubes
Monarch® Spin Columns S1A and Tubes
Monarch® Spin Columns S2A and Tubes
Monarch® Spin Columns S2B and Tubes
Monarch® Spin Columns S2C and Tubes
Monarch® Spin Columns S2D and Tubes
Monarch® Spin Columns S3A and Tubes
Monarch® Spin DNA Gel Extraction Kit
Monarch® Spin gDNA Extraction Kit
Monarch® Spin High-Capacity DNA Cleanup Kit (100 μg)
Monarch® Spin PCR & DNA Cleanup Kit (5 μg)
Monarch® Spin Plasmid Miniprep Kit
Monarch® Spin RNA Cleanup Kit (10 μg)
Monarch® Spin RNA Cleanup Kit (50 μg)
Monarch® Spin RNA Cleanup Kit (500 μg)
Monarch® Spin RNA Isolation Kit (Mini)
Monarch® DNA Capture Beads
Magnetic mRNA Isolation Kit
Nuclease-free Water
Oligo d(T)25 Magnetic Beads
PURExpress® Δ Ribosome Kit
Monarch® Spin Columns S3B and Tubes
Monarch® Spin High-Capacity RNA Cleanup Kit (3 mg)
β-Agarase I
Plasmid Purification
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® Buffer B1
Monarch® Buffer B2
Monarch® Buffer B3
Monarch® Buffer BZ
Monarch® Buffer WZ
Monarch® Plasmid Miniprep Columns
Monarch® Spin Columns S2D and Tubes
Monarch® Spin Plasmid Miniprep Kit
PCR & DNA Cleanup
Exo-CIP™ Rapid PCR Cleanup Kit
Exo-CIP™ Rapid PCR Cleanup Mix
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® Buffer BZ
Monarch® Buffer WZ
Monarch® DNA Wash Buffer
Monarch® Mag Beads M2
Monarch® Mag PCR & DNA Cleanup Kit (5 μg)
Monarch® Spin Columns S3A and Tubes
Monarch® Spin High-Capacity DNA Cleanup Kit (100 μg)
Monarch® Spin PCR & DNA Cleanup Kit (5 μg)
DNA Gel Extraction
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® Buffer BY
Monarch® Buffer WZ
Monarch® DNA Wash Buffer
Monarch® Spin DNA Gel Extraction Kit
β-Agarase I
Genomic DNA Extraction & Purification
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® 2 ml Tubes
Monarch® Bead Retainers
Monarch® gDNA Binding Buffer
Monarch® gDNA Blood Lysis Buffer
Monarch® gDNA Cell Lysis Buffer
Monarch® gDNA Elution Buffer
Monarch® gDNA Elution Buffer II
Monarch® gDNA Nuclei Prep & Lysis Buffer Pack
Monarch® gDNA Tissue Lysis Buffer
Monarch® gDNA Wash Buffer
Monarch® Pestle Set
Monarch® Precipitation Enhancer
Monarch® RNase A
Monarch® Spin Columns S2C and Tubes
Monarch® Spin gDNA Extraction Kit
Monarch® DNA Capture Beads
High Molecular Weight DNA Extraction
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® 2 ml Tubes
Monarch® gDNA Elution Buffer II
Monarch® gDNA Nuclei Prep & Lysis Buffer Pack
Monarch® gDNA Wash Buffer
Monarch® HMW DNA Extraction Kit for Cells & Blood
Monarch® HMW DNA Extraction Kit for Tissue
Monarch® HMW gDNA Tissue Lysis Buffer
Monarch® Pestle Set
Monarch® Precipitation Enhancer
Monarch® Protein Separation Solution
Monarch® RBC Lysis Buffer
Monarch® RNase A
Monarch® Spin Columns S2C and Tubes
Monarch® DNA Capture Beads
Total RNA Extraction & Purification
Magnetic mRNA Isolation Kit
Monarch® Collection Tubes II
Monarch® DNase I, Lyophilized
Monarch® Pestle Set
Monarch® Spin RNA Isolation Kit (Mini)
Nuclease-free Water
Oligo d(T)25 Magnetic Beads
PURExpress® Δ Ribosome Kit
RNA Cleanup
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® Buffer BX
Monarch® Buffer WX
Monarch® Spin Columns S1A and Tubes
Monarch® Spin Columns S2A and Tubes
Monarch® Spin Columns S2B and Tubes
Monarch® Spin RNA Cleanup Kit (10 μg)
Monarch® Spin RNA Cleanup Kit (50 μg)
Monarch® Spin RNA Cleanup Kit (500 μg)
Monarch® Spin Columns S3B and Tubes
Monarch® Spin High-Capacity RNA Cleanup Kit (3 mg)
Magnetic Bead-Based Nucleic Acid Purification
Monarch StabiLyse™ DNA/RNA Buffer
Monarch® Buffer BX
Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit
Monarch® Mag Cell-free RNA (cfRNA) Extraction Kit
Monarch® Mag PCR & DNA Cleanup Kit (5 μg)
Monarch® Mag Viral DNA/RNA Extraction Kit
