NEBNext UltraShear® Long Read
Fragmenting genomic DNA (gDNA) is key to improving long-read sequencing performance and data yield. NEBNext UltraShear® Long Read is a fast, flexible enzymatic method for gDNA fragmentation, designed for use upstream of Oxford Nanopore Technologies® (ONT) and PacBio® library preparation and sequencing. This time-tunable solution allows precise fragment sizing from 2 kb to 30 kb in as little as 30 minutes, while preserving native methylation marks. It works with a broad input range (250 ng–5 µg) and is compatible with automation, making it easy to scale as sequencing needs grow. NEBNext UltraShear Long Read provides a cost-effective and reliable alternative to mechanical shearing methods.
- Improve your sequencing yield while maintaining high N50 values
- Achieve unbiased fragmentation from 2–30 kb for diverse long-read applications
- Preserve native methylation for downstream epigenetic analyses
- Scale effortlessly with an automation-ready process and flexible input range (250 ng–5 µg)
- Cut costs and simplify workflows by eliminating specialized instruments and consumables
Note: This product is intended to support long-read library preparation and sequencing applications. For enzymatic DNA fragmentation in short-read sequencing workflows please see NEBNext UltraShear® (NEB #M7634).
Product Information
NEBNext UltraShear Long Read contains reagents to enzymatically fragment gDNA in a time- and temperature-dependent manner generating DNA fragment lengths ranging between 2 kb to 30 kb. These gDNA fragment lengths are compatible with different long-read applications, including the current commercially available platforms from ONT and PacBio.
Like its short-read counterpart NEBNext UltraShear (NEB #M7634), fragmentation with NEBNext UltraShear Long Read maintains methylation marks, making it compatible with methylation analysis workflows. As an alternative to mechanical fragmentation methods like Covaris® gTUBE™, it offers a fast, scalable, user- and automation-friendly workflow requiring minimal hands-on time.
More data supporting the claims made about the NEBNext UltraShear Long Read product can be found on the E3430 Supplemental Data Page.
This product is related to the following categories:
- Library Preparation for Oxford Nanopore Technologies,
- DNA Fragmentation,
- Next Generation Sequencing Library Preparation
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| E3430S | NEBNext UltraShear® Long Read | E3430-2 | -20 | ||
| NEBNext UltraShear® LR Enzyme Mix | E3431AVIAL | -20 | 1 x 0.068 ml | Not Applicable | |
| NEBNext UltraShear® LR Reaction Buffer | E3432AVIAL | -20 | 1 x 0.476 ml | Not Applicable | |
| NEBNext UltraShear® LR Supplement | E3433AVIAL | -20 | 1 x 0.102 ml | Not Applicable | |
| TE Buffer | E3434AVIAL | -20 | 1 x 6.3 ml | 1 X | |
| NEBNext® Sample Purification Beads | E3435S | 25 | |||
| NEBNext® Sample Purification Beads | E3435AVIAL | 25 | 1 x 2.67 ml | Not Applicable |
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| E3430L | NEBNext UltraShear® Long Read | E3430-3 | -20 | ||
| NEBNext UltraShear® LR Enzyme Mix | E3431AAVIAL | -20 | 1 x 0.22 ml | Not Applicable | |
| NEBNext UltraShear® LR Reaction Buffer | E3432AAVIAL | -20 | 2 x 0.778 ml | Not Applicable | |
| NEBNext UltraShear® LR Supplement | E3433AAVIAL | -20 | 1 x 0.333 ml | Not Applicable | |
| TE Buffer | E3434AAVIAL | -20 | 1 x 22.5 ml | 1 X | |
| NEBNext® Sample Purification Beads | E3435L | 25 | |||
| NEBNext® Sample Purification Beads | E3435AAVIAL | 25 | 1 x 9.79 ml | Not Applicable |
Related Products
Companion Products
- Monarch® HMW DNA Extraction Kit for Cells and Blood
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing
- NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing
- NEBNext® Magnetic Separation Rack
- Monarch® HMW DNA Extraction Kit for Tissue
Protocols, Manuals & Usage
Protocols for this product
- Where can I find protocols for use with NEBNext UltraShear Long Read?
Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
- manualE3430
FAQs & Troubleshooting
FAQs
- Are there any recommendations for handling High Molecular Weight gDNA (HMW gDNA) Samples prior to use with NEBNext UltraShear® Long Read?
- Can I fragment my gDNA sample with NEBNext UltraShear® Long Read in buffers other than 1X TE (10mM Tris pH 8.0, 1mM EDTA)?
- Do you really need to vortex the NEBNext UltraShear® LR Enzyme Mix?
- Following the fragmentation step of the NEBNext UltraShear® Long Read protocol, can the reactions be stored at 4˚C?
- Is NEBNext UltraShear® Long Read automation friendly?
- Is NEBNext UltraShear® Long Read the same thing as NEBNext UltraShear?
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
- E3430S_L_G_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- E3430L_E3435L_v1_10335905
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
- NEBNext UltraShear® Long Read
- NEBNext UltraShear® LR Enzyme Mix
- NEBNext UltraShear® LR Reaction Buffer
- NEBNext® Sample Purification Beads
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner.
