Protein Tools
Product Overview
New England Biolabs offers an array of protein tools for all of your protein analysis needs.
Proteome Analysis
Trypsin-digested BSA MS Standard (CAM-modified) (NEB #P8108) is a complex mixture of peptides that can be used to calibrate mass spectrometers.
Proteases
NEB offers several proteases for proteomics applications, including Trypsin (NEB #P8119), AspN (NEB #P8104), GluC (NEB #P8100), Furin (NEB #P8077), and Endoproteinase LysC (NEB #P8109).
Protein Labeling
New England Biolabs provides the SNAP-tag®, CLIP-tag™ and ACP/MCP-tag protein labeling systems that facilitate the attachment of virtually any molecule to a protein of interest, and can be used for studying the function and localization of proteins in living and fixed cells, as well as protein immobilization.
Phage Display
The Ph.D.™ libraries from New England Biolabs have become the preeminent tools in this field, with hundreds of publications describing applications, including epitope mapping/vaccine development, mapping protein-protein contacts and identification of peptide mimics of non-peptide ligands. Bioactive peptides, which can be used as cell-targeting or gene delivery agents, have been identified either by panning against purified receptors or against intact cells or tissue samples, both in vitro and in vivo. NEB offers displayed peptide libraries of 7 (NEB #E8211S) and 12 (NEB #E8210S) residues, as well as a disulfide-contrained 7-residue library (NEB #E8212S).
Protein Kinases
The reversible addition of phosphate groups to proteins is important for the transmission of signals within eukaryotic cells and, as a result, protein phosphorylation and dephosphorylation regulate many diverse cellular processes. As the number of known protein kinases has increased at an ever-accelerating pace, it has become more challenging to determine which protein kinases interact with which substrates in the cell. The determination of consensus phosphorylation site motifs by amino acid sequence alignment of known substrates has proven useful in this pursuit. These motifs can be helpful for predicting phosphorylation sites for specific protein kinases within a potential protein substrate. NEB offers a line of several protein kinases to aid in this research.
Protein Phosphatases
Protein phosphorylation plays a role in the regulation of the function and activity of protein factors and enzymes. Protein phosphorylation can be found on tyrosine, histidine, serine and threonine residues. Analysis of the presence of such phosphorylation, and its attendant effects, is often aided by removal of the protein phosphate groups by phosphatases. NEB offers several protein phosphatases that remove phosphate groups from different subsets of these residues.
Protein Standards
New England Biolabs offers a selection of highly pure protein standards. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored bands for easy identification. Standards are provided pre-mixed with loading buffer and reducing agent.
Protein Tools includes these subcategories:
FAQs for Protein Tools
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What is Ph.D.™ Phage Display?
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What products has NEB developed from ongoing basic research in molecular parasitology?
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Which residues does Endoproteinase AspN cut?
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Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
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How does one do a Trypsin in-gel digest?
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I can’t get Endoproteinase AspN to digest my protein.
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I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
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What peptide libraries are available for use with Ph.D.™ Phage Display?
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What is the Proteinase K activity in commonly used buffers?
Protocols for Protein Tools
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In-Solution Digestion Using Trypsin-ultra™, Mass Spectrometry Grade (Recombinant) (NEB #P8119)
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Filtration Assisted Sample Preparation (FASP) Using Trypsin-ultra™, Mass Spectrometry Grade (Recombinant) (NEB #P8119)
Application Notes for Protein Tools
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Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
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Ph.D.™ Peptide Display Cloning System
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Simultaneous Fluorescent Labeling of Proteins in Living Cells
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Intein-Mediated Protein Ligation IPL and Labeling with the IMPACT™ Kit
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Comprehensive structural and positional profiling of intact complex O-glycans in biologic drugs using O-Glycoprotease (IMPa)
Tools & Resources
Feature Articles
Understand the common types of parasitic infections in humans and why they are still increasing in tropical regions.
Brochures
Posters
Protease Selection Chart
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Product Listing
New England Biolabs offers proteases, phosphatases, kinases, chitinases and protein markers and ladders, as well as tools for phage display and protein labeling.
