Genome Editing with CRISPR/Cas
Product Overview
CRISPR-Cas-based techniques enable precise gene editing workflows, enrichment approaches and in vitro detection assays with easy retargeting. A Cas ribonucleoprotein (RNP) complex activates when its guide RNA binds to a target sequence and its Cas-interacting domain binds to a specific protospacer-adjacent motif (PAM) in a nucleic acid substrate. Delivery formats may incorporate plasmid constructs, viral vectors, mRNA molecules, sgRNA molecules and Cas proteins.
A toolbox to optimize CRISPR-Cas workflows
NEB offers highly characterized Cas nucleases and unique solutions for streamlined RNA synthesis, cloning, and validation of CRISPR-based genome editing. Each Cas endonuclease is characterized for use in common CRISPR applications. For example, EnGen® Spy Cas9 HF1 (NEB #M0667) is recommended for reduced off-targeting in vivo, EnGen SpRY Cas9 (NEB #M0669) is recommended to expand editable regions in vivo and abolish PAM requirements in vitro and EnGen Lba Cas12a (Cpf1) (NEB #M0653) opens up editing in additional genomic regions and ectothermic organisms. NEBuilder® HiFi DNA Assembly provides exceptional cloning efficiency to streamline the construction of Cas-sgRNA expression vectors, single sgRNA templates, or sgRNA libraries. Our extensive RNA Synthesis and Modification portfolio supports the synthesis of sgRNA and Cas mRNA. Analysis of genome editing results can progress to validation using our solutions for simple enzymatic detection assays, Sanger sequencing-based detection and next-generation sequencing with NEBNext® Ultra library prep kits.
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Feature Articles
CRISPR/Cas9 & Targeted Genome Editing: New Era in Molecular Biology
Understand the history, importance and future of CRISPR/Cas9 and target genome editing
Minding your caps and Poly A tails – Strategies for synthesizing in vitro transcribed (IVT) mRNA
Understand options for selecting reagents and the extent of reagent influence on synthesized mRNA cap functionality.
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Product Listing
NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.
