RNA Synthesis and Modification

Product Overview​

RNA plays a multitude of cellular roles in all kingdoms of life and is a promising area of research in biotechnology and therapeutic development. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression. Our understanding of RNAs as regulatory molecules in the cell has dramatically changed over the last decade. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology. NEB’s RNA reagents can be ordered in bulk, GMP-grade*, or customized formats for advanced research and development projects, please contact our Customized Solutions team for more information.

NEB provides in vitro transcription (IVT) reagents from bench-scale to commercial-scale

NEB offers a range of products for RNA research, including reagents for synthesis, extraction, purification, cleanup, analysis, modification, and detection of RNA molecules. These products are available from bench-scale to commercial-scale to serve both academic and industrial needs. Further, we provide these products at quality levels that support vaccine and therapeutic manufacturing. Experience improved performance and increased yields, enabled by our expertise in enzymology.

RNA Synthesis and Modification includes these subcategories:

RNA Capping
RNA Extraction and Purification
RNA Ligation
RNA Markers and Ladders
RNA Modification
RNA Synthesis In vitro Transcription (IVT)
RNase Inhibitors
RNases

Protocols for RNA Synthesis and Modification

  • Protocol for ChIP DNA (E7335)

Application Notes for RNA Synthesis and Modification

  • Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

Tools & Resources

Feature Articles

  • Molecular Cloning Technology - Past, Present and Future

    Read about the evolution of cloning technology—from early recombinant DNA experiments to advanced molecular cloning techniques—and learn how these innovations revolutionized genetics, biotechnology, and biomedical research.

Brochures

  • GMP-Grade Products Brochure
  • RNA Synthesis Brochure
  • RNA Technical Guide

Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Featured Products
  • HiScribe® T7 mRNA Kit with CleanCap® Reagent AG
  • HiScribe® T7 High Yield RNA Synthesis Kit
  • Faustovirus Capping Enzyme
  • Vaccinia Capping System
  • mRNA Cap 2´-O-Methyltransferase
  • EnGen® sgRNA Synthesis Kit, S. pyogenes
  • DNase I-XT

RNA Synthesis and Modification

RNA Capping
RNA Extraction and Purification
RNA Ligation
RNA Markers and Ladders
RNA Modification
RNA Synthesis In vitro Transcription (IVT)
RNase Inhibitors
RNases

RNA Capping

3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog
Faustovirus Capping Enzyme
G(5')ppp(5')A RNA Cap Structure Analog
G(5')ppp(5')G RNA Cap Structure Analog
HiScribe® SP6 RNA Synthesis Kit
HiScribe® T7 ARCA mRNA Kit
HiScribe® T7 ARCA mRNA Kit (with tailing)
HiScribe® T7 High Yield RNA Synthesis Kit
HiScribe® T7 mRNA Kit with CleanCap® Reagent AG
m7G(5')ppp(5')A RNA Cap Structure Analog
mRNA Cap 2´-O-Methyltransferase
mRNA Cap N6 Methyltransferase (m6A)
Vaccinia Capping System

RNA Extraction and Purification

Magnetic mRNA Isolation Kit
Monarch® Buffer BX
Monarch® Buffer WX
Monarch® Collection Tubes II
Monarch® gDNA Wash Buffer
Monarch® Pestle Set
Monarch® Spin Columns S1A and Tubes
Monarch® Spin Columns S2A and Tubes
Monarch® Spin Columns S2B and Tubes
Monarch® Spin RNA Cleanup Kit (10 μg)
Monarch® Spin RNA Cleanup Kit (50 μg)
Monarch® Spin RNA Cleanup Kit (500 μg)
Monarch® Spin RNA Isolation Kit (Mini)
Nuclease-free Water
Oligo d(T)25 Magnetic Beads

RNA Ligation

5´ DNA Adenylation Kit
RtcB Ligase
SplintR® Ligase
T4 RNA Ligase 1 (ssRNA Ligase)
T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2, truncated
T4 RNA Ligase 2, truncated K227Q
T4 RNA Ligase 2, truncated KQ
T4 RNA Ligase Reaction Buffer
Thermostable 5´ App DNA/RNA Ligase

RNA Markers and Ladders

dsRNA Ladder
Low Range ssRNA Ladder
microRNA Marker
RNA Loading Dye, (2X)
ssRNA Ladder

RNA Modification

3´-Biotin-GTP
3´-Desthiobiotin-GTP
5´ Deadenylase
5-Methoxy-Uridine-5´-Triphosphate (5-Methoxy-UTP)
5-Methyl-Cytidine-5´-Triphosphate (5-Methyl-CTP)
Antarctic Phosphatase
Apyrase
E. coli Poly(A) Polymerase
E. coli RNA Polymerase, Holoenzyme
EpiMark® N6-Methyladenosine Enrichment Kit
FTO RNA Demethylase
Hydrophilic Streptavidin Magnetic Beads
Micrococcal Nuclease
mRNA Decapping Enzyme
Mung Bean Nuclease
N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)
Nucleoside Digestion Mix
NudC Pyrophosphatase
Pseudouridine-5´-Triphosphate (Pseudo-UTP)
Pyrophosphatase, Inorganic (E. coli)
Pyrophosphatase, Inorganic (yeast)
Quick CIP
RNA 5' Pyrophosphohydrolase (RppH)
RNA Inosine Endonuclease
RNase 4 Digestion and 3´ End Repair Mix
RNase R
Sce PUS1
T4 Polynucleotide Kinase (3' phosphatase minus)
Thermostable Inorganic Pyrophosphatase
XRN-1
yDcpS

RNA Synthesis In vitro Transcription (IVT)

3´-Biotin-GTP
3´-Desthiobiotin-GTP
5-Methoxy-Uridine-5´-Triphosphate (5-Methoxy-UTP)
5-Methyl-Cytidine-5´-Triphosphate (5-Methyl-CTP)
DNase I (RNase-free)
DNase I-XT
E. coli Poly(A) Polymerase
E. coli RNA Polymerase, Core Enzyme
E. coli RNA Polymerase, Holoenzyme
HiScribe® SP6 RNA Synthesis Kit
HiScribe® T7 ARCA mRNA Kit
HiScribe® T7 ARCA mRNA Kit (with tailing)
HiScribe® T7 High Yield RNA Synthesis Kit
HiScribe® T7 mRNA Kit with CleanCap® Reagent AG
HiScribe® T7 Quick High Yield RNA Synthesis Kit
Hi-T7® RNA Polymerase
Hi-T7® RNA Polymerase (High Concentration)
m7G(5')ppp(5')G RNA Cap Structure Analog
N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)
Poly(U) Polymerase
Pseudouridine-5´-Triphosphate (Pseudo-UTP)
Pyrophosphatase, Inorganic (E. coli)
Pyrophosphatase, Inorganic (yeast)
Ribonucleotide Solution Set
Ribonucleotide Solution Mix
RNase Inhibitor, Human Placenta
RNase Inhibitor, Murine
RNase Inhibitor, Murine (Glycerol-free)
SP6 RNA Polymerase
T3 RNA Polymerase
T7 RNA Polymerase
T7 RNA Polymerase (High Concentration)

RNase Inhibitors

Ribonucleoside Vanadyl Complex
RNase Inhibitor, Human Placenta
RNase Inhibitor, Murine
RNase Inhibitor, Murine (Glycerol-free)

RNases

Exonuclease T
Monarch® RNase A
RNase 4
RNase 4 Digestion and 3´ End Repair Mix
RNase H
RNase HII
RNase If
RNase R
ShortCut® RNase III
Thermostable RNase H
XRN-1