Competent Cells
Product Overview
Cloning Strains
For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.
Yeast
NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.
E. coli for Protein Expression
NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.
Competent Cells includes these subcategories:
FAQs for Competent Cells
- Can I store competent cells at -20°C instead of -80°C?
- Does New England Biolabs offer a methyltransferase free strain of Competent E.coli?
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- What are the advantages of New England Biolabs' competent cells?
- What does the growth curve of NEB Turbo cells look like compared to DH5α?
- What is LysY?
- What volume of DNA can be added into competent cells?
- Which kind of transformation tubes should be used?
- What applications are SHuffle® strains useful for?
- Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?
- Which strain of Competent E.coli should I use for general cloning?
Protocols for Competent Cells
- Optimizing Restriction Endonuclease Reactions
Application Notes for Competent Cells
- Protein Expression with T7 Express Strains
Tools & Resources
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Competent Cell Product Comparison
- Competent Cell Selection Guide
- Characteristics of Select E.coli Strains
- Troubleshooting Transformation Reactions
- Restriction of Foreign DNA by E. coli K-12
- Additional E. coli Strain Genotypes
- McrA, McrBC and EcoKI Strain Phenotypes
- Chemical Transformation Tips
- Competent E. coli Genome Sequences Tool
- Making Unmethylated (Dam- Dcm-) DNA
- Electroporation Tips
- Genetic Markers
Publications related to Competent Cells
Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli
J Biol Chem; 294(48), 18046-18056.
PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
Tuning Escherichia coli for membrane protein overexpression
Proc Natl Acad Sci U S A; 105, 14371-14376.
Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli.
Protein Expr Purif; 153, 7-17.
PubMedID: 30081196, DOI: 10.1016/j.pep.2018.08.001.
Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote
Redox Biol; 26, 101280.
PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
Use of the Shuffle Strains in Production of Proteins.
Curr Protoc Protein Sci; Aug 1, 1;85:5.26.1-5.26.21.
PubMedID: 27479507, DOI: 10.1002/cpps.11.
Aeropyrum pernix membrane protein VKOR promotes protein disulfide bond formation in two subcellular compartments.
Microbiology; 163 (12), 1864-1869.
PubMedID: 291309344
Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli.
mAbs; 10 (7), 992-1002.
PubMedID: 30060704, DOI: 10.1080/19420862.2018.1496879
Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions
Protein Sci; 20, 51-61.
Revolutionizing membrane protein overexpression in bacteria
Microb Biotechnol; 3, 403-411.
NEB Competent E. coli Strain Selection
NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Product Listing
New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress® products, resulting from our 50+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.
