Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)
- Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- 2X master mix containing Bst-XT WarmStart DNA Polymerase and WarmStart® RTx Reverse Transcriptase
- Bst-XT combines the high specificity of Bst 2.0 and the polymerization speed of Bst 3.0 DNA Polymerases
- Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the mix
- Fully buffered master mix is compatible with different sample types and supports multiple detection methods
- WarmStart technology inhibits enzyme activity at room temperature
- Broad optimal reaction temperature from 50-70°C
- View the Bst-XT: An improved Bst DNA polymerase to support loop mediated isothermal amplification NEB TV webinar
Featured Video
Loop Mediated Isothermal Amplification (LAMP) Tutorial
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| Catalog # | Concentration | Size |
|---|---|---|
| M1712S | 2 X | 100 reactions |
| M1712L | 2 X | 500 reactions |
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Product Information
Bst-XT WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) provides a one-step solution for amplification of DNA (LAMP) or RNA (RT-LAMP) targets. It features a blend of Bst-XT WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized, fully buffered master mix. Bst-XT WarmStart DNA Polymerase is an engineered variant of Bacillus stearothermophilus DNA Polymerase, Large Fragment fused to a novel nucleic acid binding domain for improved isothermal amplification performance. Bst-XT combines the high specificity of Bst 2.0 and the fast polymerization speed of Bst 3.0 DNA polymerases. WarmStart RTx Reverse Transcriptase is a unique, in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits enzyme activity below 40°C.
Comparison of LAMP master mix products
| Feature | WarmStart® LAMP Kit (DNA & RNA) NEB #E1700 |
WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) NEB #E1708 |
WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) NEB #M1708 |
Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) NEB #M1712 |
|---|---|---|---|---|
| DNA polymerase | Bst 2.0 WarmStart | Bst 2.0 WarmStart | Bst 2.0 WarmStart | Bst-XT WarmStart |
| Reverse transcriptase | WarmStart RTx | WarmStart RTx | WarmStart RTx | WarmStart RTx |
| LAMP fluorescent dye | Provided | Provided | Sold separately | Sold separately |
| Amplification speed | ★★★ | ★★★ | ★★★ | ★★★★★ |
| Specificity | ★★★★★ | ★★★★★ | ★★★★★ | ★★★★★ |
| Carryover contamination prevention | No | Yes | Yes | Yes |
| Room temperature set-up | Enabled | Enabled | Enabled | Enabled |
| Optimal LAMP temperature | 60-70°C | 60-70°C | 60-70°C | 50-70°C |
★★★★★ = optimal, recommended product for selected application
★★★ = will perform selected application
The mix is compatible with different sample types and enables multiple detection methods including real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization such as colorimetric detection via a metal sensing dye (e.g., calcein.)
The inclusion of dUTP and thermolabile UDG reduces the possibility of carryover contamination, where unintended product of a previous amplification serves as the substrate of a subsequent reaction. Thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on the reaction.
This product is related to the following categories:
- Isothermal Amplification & Strand Displacement
This product can be used in the following applications:
- Isothermal Amplification
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| M1712S | Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) | M1712SVIAL | -20 | 1 x 1.25 ml | 2 X |
| M1712L | Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) | M1712SVIAL | -20 | 5 x 1.25 ml | 2 X |
Properties & Usage
Materials Required but not Supplied
Heating Device: heat block, water bath, or real-time fluorimeter (qPCR instrument) set to 65°C
Protocols, Manuals & Usage
Protocols for this product
- Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) (NEB #M1712)
Application Notes
- LAMP Primer Design using the NEB LAMP Primer Design Tool: Critical Considerations for Assay Robustness, Speed and Sensitivity
Tools & Resources
Web Tools
- NEB LAMP Primer Design Tool
FAQs & Troubleshooting
FAQs
- What is the Mg++ concentration in the Bst-XT LAMP/RT-LAMP Master Mix?
- What is the optimal LAMP/RT-LAMP amplification temperature while using Bst-XT LAMP/RT-LAMP Master Mix?
- Does Bst-XT WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) enable carryover prevention/contamination reduction?
- How fast should I expect a result when using the Bst-XT LAMP/RT-LAMP Master Mix?
- What detection methods are compatible with the Bst-XT LAMP/RT-LAMP Master Mix?
- What are the advantages of using standard LAMP (NEB #M1712, E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
- Can I set up my LAMP/RT-LAMP reactions at room temperature?
- How do I design LAMP Primers?
- Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
- Is a separate incubation step required for RT-LAMP?
- What type of purification is recommended for LAMP primers?
- The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
Citations & Technical Literature
Citations
Product Citation Tool
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Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
- M1712S_L_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- M1712L_v1_10316498
- M1712S_v1_10316496
- M1712L_v1_10336171
- M1712S_v1_10336172
- M1712L_v1_10348588
- M1712L_v1_10353012
- M1712S_v1_10349060
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
- Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
