DNA Modifying Enzymes & Cloning Technologies

Product Overview​

Advances in molecular biology require constant innovation and forward progress in the tools and techniques at the core of the field. New England Biolabs is committed to providing a wide variety of essential reagents that serve to advance the frontier of DNA-based manipulations.

NEB’s enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value

DNA Modifying Enzymes & Cloning Technologies includes these subcategories:
Argonautes
Closed-ended DNA & Protelomerases
DNA Ligase
DNA Repair Enzymes and Structure-specific Endonucleases
Base Modifying Enzymes
Exonucleases and Non-specific Endonucleases
Phosphatase
Recombinase
ssDNA Binding Protein
Kinase
DNA Labeling
Other
Protocols for DNA Modifying Enzymes & Cloning Technologies
Sample preparation using ssRNA Ladder provided buffer (NEB #N0364)
Optimizing Restriction Endonuclease Reactions
NEBuilder® HiFi DNA Assembly Electrocompetent Transformation Protocol
NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol (E2621, E5520, E2623)
HiFi Taq DNA Ligase (M0647) Protocol
Protocol for a substrate with 5-hydroxymethyluridine such as bacteriophage SP8 genomic DNA (M0659)
Supercoiling DNA Using DNA Gyrase (E. coli) (NEB #M7636)
EnClose® TelA Protelomerase Reaction Protocol (NEB #M0621)
Deamination of Single Stranded DNA (ssDNA) Using APOBEC3A (NEB #M0648)
Oxidation of Methylated Cytosine Using TET2 (NEB #M1524)
Application Notes for DNA Modifying Enzymes & Cloning Technologies
Joining of Difficult to Ligate dsDNA Fragments with Blunt TA Ligase Master Mix
Tools & Resources

Feature Articles

Molecular Cloning Technology - Past, Present and Future

Read about the evolution of cloning technology—from early recombinant DNA experiments to advanced molecular cloning techniques—and learn how these innovations revolutionized genetics, biotechnology, and biomedical research.

Brochures

Choosing the Right Exonuclease Poster

Selection Tools

Properties of DNA and RNA Ligases
DNA Ligase Selection Chart

Troubleshooting Guides

Troubleshooting Guide for Cloning

Usage Guidelines

Traditional Cloning Quick Guide
Tips for Maximizing Ligation Efficiencies

Posters

Application of a fully synthetic Golden Gate assembly system to the rapid and flexible engineering of Pseudomonas aeruginosa phage ɸKMV
Fully synthetic chassis for the rapid engineering of Mycobacteriophages with therapeutic potential
miRNA Detection by Ligation and Amplification of Complementary DNA Oligos Using SPlintR® Ligase (2015)
Single-Strand Specific, Plasmid Borne DNA Methyltransferases M.BceJIII and M.EcoGIX Regulate Plasmid and Single-Stranded Phage Replication. (2017)
Choosing the Right Exonuclease (2019)
Genome sequences of the human filarial parasites Mansonella perstans and Mansonella ozzardi
Genomes of the Wolbachia endosymbionts from the human filarial parasites Mansonella perstans and Mansonella ozzardi reveal multiple origins of nematode-Wolbachia symbiosis
Application of High Complexity Golden Gate Assembly to Rapid Engineering of Bacteriophage Genomes
Prediction of Golden Gate Assembly GGA Using a Comprehensive Analysis of T4 DNA Ligase End-Joining Fidelity and Bias (2018)
EnGen® SpRY Cas9 – A universal tool for DNA cleavage in vitro
Rapid DIY Gene Synthesis
Publications related to DNA Modifying Enzymes & Cloning Technologies
Callahan, S.J., Luyten, Y.A., Gupta, Y.K., Wilson, G.G., Roberts, R.J., Morgan, R.D. and Aggarwal, A.K. (2016) Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. PLoS Biol; 14(4):e1002442. PubMedID: 27082731
Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. (2016) Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res; 44(19):9413-9425. PubMedID: 27580720
Blow, M.J., Clark, T.A., Daum, C.G., Deutschbauer, A.M., Fomenkov, A., Fries, R., Froula, J., Kang, D.D., Malmstrom, R.R., Morgan, R.D., Posfai, J., Singh, K., Visel, A., Wetmore, K., Zhao, Z., Rubin, E.M., Korlach, J., Pennacchio, L.A. and Roberts, R.J. (2016) The Epigenomic Landscape of Prokaryotes. PLoS Genet; 12(2):e1005854. PubMedID: 26870957. DOI: 10.1371/journal.pgen.1005854
Ee, R., Lim, Y.L., Yin, W.F., See-Too, W.S., Roberts, R.J. and Chan, K.G. (2016) Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25(T) gen. nov., sp. nov. Front Microbiol; 7:1362. PubMedID: 27630623. DOI: 10.3389/fmicb.2016.01362
Yao, K., Muruvanda, T., Roberts, R.J., Payne, J., Allard, M.W. and Hoffmann, M. (2016) Complete Genome and Methylome Sequences of Salmonella enterica subsp. enterica Serovar Panama and Serovar Sloterdijk. Genome Announc; 4(2). PubMedID: 26988049
Anton, B.P., Harhay, G.P., Smith, T.P., Blom, J. and Roberts, R.J. (2016) Comparative Methylome Analysis of the Occasional Ruminant Respiratory Pathogen Bibersteinia trehalosi. PLoS One; 11(8):e0161499. PubMedID: 27556252
Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. (2015) Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One; 10(4):e0123639. PubMedID: 25860355
Seib, K.L., Jen, F.E., Tan, A., Scott, A.L., Kumar, R., Power, P.M., Chen, L.T., Wu, H.J., Wang, A.H., Hill, D.M., Luyten, Y.A., Morgan, R.D., Roberts, R.J., Maiden, M.C., Boitano, M., Clark, T.A., Korlach, J., Rao, D.N. and Jennings, M.P. (2015) Specificity of the ModAll, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria Meningitidis Nucleic Acids Res; 43(8):4150-4162. PubMedID: 25845594. DOI: 10.1093/nar/gkv219
Xu, S-Y., Boitano, M., Clark, T.A., Vincze, T., Fomenkov, A., Kumar, S., Too, PH-M., Gonchar, D. and Degtyarev, S.K. (2015) Complete Genome Sequence Analysis of Bacillus Subtilis T30 Genome Announc; 3(3):e00395-15. PubMedID: 25953183
Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. (2015) Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc; 3(1). PubMedID: 25700417
Anton, B.P., Mongodin, E.F., Agrawal, S., Fomenkov, A., Byrd, D.R., Roberts, R.J. and Raleigh, E.A. (2015) Complete Genome Sequence of ER2796, a DNA Methyltransferase- Deficient Strain of Escherichia coli K-12 PLoS One; 10(5):e0127446. PubMedID: 26010885
Deptula, P., Smolander, O.-P., Laine, P., Roberts, R.J., Edelmann, M., Peltola, P., Piironen, V., Paulin, L., Storgards, E., Savijoki, K., Laitila, A., Auvinen, P., Varmanen, P. (2018) Acidipropionibacterium virtanenii sp nov isolated from malted barley Int J Syst Evol Microbiol; 68:3175-3183. PubMedID: 30156530
Anton, B.P., Roberts, R.J., Fomenkov, A., Humbert, A., Stoian, N., Zeilstra-Ryalls, J. (2018) Complete genome sequences of two Rhodobacter strains Microbiol Resour Announc; 7(12):e01162-18. PubMedID: 30533667
Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. (2018) Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414 Genome Announ.; 6(6):e01605-17. PubMedID: 29439055. DOI: 10.1128/genomeA.01605-17
Bottacini, F., Morrissey, R., Roberts, R.J., James, J., van Breen, J., Egan, M., Lambert, J., van Limpt, K., Knol, J., O'Connell Motherway, M. and van Sinderen, D. (2018) Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve. Nucleic Acids Res; 46(4):1860-1877. PubMedID: 29294107. DOI: 10.1093/nar/gkx1289
Murray, Iain; Morgan, Rick; Luyten, Yvette; Fomenkov, Alexey; Correa, Ivan; Dai, Nan; Roberts, Rich. (2018) The non-specific adenine DNA methyltransferase M.EcoGII. Nucleic Acids Res; 840-848. PubMedID: 29228259. DOI: 10.1093/nar/gkx1191
Mauris, J. and Evans, T.C., Jr. (2010) A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem; 285(15):11087-11092. PubMedID: 20129926
Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. (2014) REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Res. PubMedID: 25378308

Featured Products

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Blunt/TA Ligase Master Mix
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Cas9 Nuclease, S. pyogenes
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Quick CIP

DNA Modifying Enzymes & Cloning Technologies

DNA Modifying Enzymes & Cloning Technologies

9°N™ Reverse Gyrase
APOBEC3A
DNA Gyrase (E. coli)
Monarch® DNase I, Lyophilized
NEBExpress® Salt Active Nuclease, GMP Grade
Nucleoside Digestion Mix
TET2

Argonautes

Tth Argonaute (TtAgo)

Closed-ended DNA & Protelomerases

EnClose® Cell-free dbDNA™ Synthesis Kit
EnClose® TelA Protelomerase
EnClose® TelN Protelomerase

DNA Ligases

9°N™ DNA Ligase
Blunt/TA Ligase Master Mix
E. coli DNA Ligase
ElectroLigase®
HiFi Taq DNA Ligase
Hi-T4™ DNA Ligase
Immobilized T4 DNA Ligase
Instant Sticky-end Ligase Master Mix
NEBNext® Quick Ligation Module
NEBridge® Ligase Master Mix
Quick Ligation™ Kit
Salt-T4® DNA Ligase
SplintR® Ligase
StickTogether™ DNA Ligase Buffer
T3 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase Reaction Buffer
T7 DNA Ligase
Taq DNA Ligase
Taq DNA Ligase Reaction Buffer
Thermostable 5´ App DNA/RNA Ligase

DNA Repair Enzymes and Structure-specific Endonucleases

Afu Uracil-DNA Glycosylase (UDG)
Antarctic Thermolabile UDG
APE 1
Authenticase®
Endonuclease III (Nth)
Endonuclease IV
Endonuclease V
Endonuclease VIII
EnGen® Mutation Detection Kit
Fpg
hAAG
hSMUG1
Mismatch Endonuclease I
PreCR® Repair Mix
RecA
T4 PDG (T4 Endonuclease V)
T7 Endonuclease I
Thermolabile USER® II Enzyme
Thermostable Endonuclease Q
Thermostable FEN1
Thermostable OGG
Thermostable USER® III Enzyme
Thymine-DNA Glycosylase (TDG)
Topoisomerase I (E. coli)
Tth Endonuclease IV
Uracil Glycosylase Inhibitor (UGI)
Uracil-DNA Glycosylase (UDG)
USER® Enzyme
WarmStart® Afu Uracil-DNA Glycosylase (UDG)

Base Modifying Enzymes

AluI Methyltransferase
APOBEC3A
BamHI Methyltransferase
CpG Methyltransferase (M.SssI)
EcoGII Methyltransferase
EcoRI Methyltransferase
GpC Methyltransferase (M.CviPI)
HaeIII Methyltransferase
Hhal Methyltransferase
HpaII Methyltransferase
MspI Methyltransferase
TaqIMethyltransferase
TET2

Exonucleases and Non-specific Endonucleases

DNase I (RNase-free)
DNase I Reaction Buffer
DNase I-XT
DNase I-XT Reaction Buffer
Duplex DNase
Exonuclease I (E. coli)
Exonuclease I Reaction Buffer
Exonuclease III (E. coli)
Exonuclease T
Exonuclease V (RecBCD)
Exonuclease VII
Exonuclease VIII, truncated
Lambda Exonuclease
Micrococcal Nuclease
Msz Exonuclease I
Mung Bean Nuclease
NEBExpress® Salt Active Nuclease
Nuclease P1
RecJf
T5 Exonuclease
T7 Endonuclease I
T7 Exonuclease
Thermolabile Exonuclease I

Phosphatases

Antarctic Phosphatase
Antarctic Phosphatase Reaction Buffer
Apyrase
Pyrophosphatase, Inorganic (E. coli)
Pyrophosphatase, Inorganic (yeast)
Quick CIP
Shrimp Alkaline Phosphatase (rSAP)
Thermostable Inorganic Pyrophosphatase

Recombinases

Cre Recombinase

ssDNA Binding Proteins

ET SSB
RecA
T4 Gene 32 Protein

Kinases

5-hydroxymethyluridine DNA Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (3' phosphatase minus)
T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

DNA Polymerase I (E. coli)
Klenow Fragment (3´→5´ exo-)
Streptavidin
T4 Polynucleotide Kinase
Terminal Transferase
Therminator™ DNA Polymerase

Other

Cryogenic Cold Storage Box
DNA Gyrase (E. coli)
EnClose® TelN Protelomerase
M13KO7 Helper Phage
β-Agarase I