RNase Inhibitor, Murine (Glycerol-free)
RNase Inhibitor, Murine (Glycerol-free)
RNase Inhibitor, Murine, specifically inhibits RNases A, B and C.
- Glycerol-free version of RNase Inhibitor, Murine (NEB #M0314) supports lyophilization and automation workflows
- Offers improved resistance to oxidation, compared to human/porcine RNase inhibitor
- Ideal for reactions where low reducing agent concentrations are required (e.g., RT-qPCR)
- Isolated from a recombinant source
- Tested for the absence of nucleases
| Catalog # | Concentration | Size |
|---|---|---|
| M0628S | 40,000 units/ml | 3,000 units |
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Product Information
RNase Inhibitor, Murine (Glycerol-free) is a 50 kDa recombinant protein of murine origin. It specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. RNase Inhibitor, Murine (Glycerol-free) is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). Recombinant Murine RNase Inhibitor lacks the cysteines identified in the human version to be sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors and is stable at low reducing agent concentrations (≤ 1 mM). This makes it ideal for reactions where low concentration reducing agent is required (e.g. Real-time RT-PCR). The RNase Inhibitor, Murine (Glycerol-free) formulation lacks glycerol, which makes it amenable to lyophilization and automation workflows.
Product Source
An E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.
This product is related to the following categories:
This product can be used in the following applications:
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| M0628S | RNase Inhibitor, Murine (Glycerol-free) | M0628SVIAL | -80 | 1 x 0.075 ml | 40,000 units/ml |
Properties & Usage
Unit Definition
One unit is defined as the amount of Murine RNase Inhibitor (Glycerol-free) required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
Advantages and Features
Application Features
- RT-PCR
- cDNA synthesis
- In vitro transcription/translation
- Enzymatic RNA labeling reaction
- Other applications where the integrity of RNA is important
Related Products
Companion Products
References
- Kim, B.M. et al. (1999). Protein Science. 8, 430-434.
Protocols, Manuals & Usage
Protocols for this product
- Using RNase Inhibitor to prevent Ribonuclease Digestion of RNA (NEB #M0314, #M0307, #M0628)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB #M0276)
FAQs & Troubleshooting
FAQs
- How many freeze/thaw cycles can RNase Inhibitor, Murine (Glycerol-free) tolerate?
- What is the difference between RNase Inhibitor, Murine, (NEB #M0314) and RNase Inhibitor, Murine (Glycerol-free), (NEB #M0628)?
- Can I use RNase Inhibitor, Murine (Glycerol-free) in PURExpress® In vitro Protein Synthesis and NEBExpress Cell-free E. coli Protein Synthesis workflows?
Tech Tips
RNase Inhibitor, Murine (Glycerol-free) is a 50 kDa protein. It will be inactive in conditions that will denature proteins.
We recommend using the inhibitor at 1unit/µl in your reaction.
The inhibitor should be added to a reaction before other components that are possible sources of RNase contamination.
The inhibitor should be used below 50°C.
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner.
