Supporting Scientists Developing Molecular Diagnostics

Product Overview​

The COVID-19 pandemic elevated many of the challenges faced by clinical labs, requiring new and innovative solutions to address them. Technology development is happening faster than ever before, exposing weaknesses in diagnostic supply chains, increasing the need for innovation and thinking differently about how diagnostics should be developed, manufactured and deployed.

Many scientists know NEB as a trusted reagent provider to the life science community. What many do not know is that we also offer products and support for scientists who are working to develop diagnostics. Extensive molecular biology and enzymology experience enable us to help customers solve the challenges inherent in technology development and ultimately in scale-up and commercialization. For scientists looking for long term, ambient-stored products, NEB’s diverse portfolio of lyophilized and lyo-compatible products, as well as custom lyophilization capabilities are also available.

Learn more about the benefits of using lyophilized reagents in our feature article: Enhancing Molecular Diagnostics in Regulated Markets with Lyophilized Assays.

It’s time to think differently

Our Customized Solutions has been serving customers for over 30 years. We understand the demands around technology development and product launches, and are able to apply a cross-functional team of scientists and staff to quickly optimize your reagent development. We have made significant investment in our scale-up and manufacturing capabilities, and can offer consistent and reliable supply chain with fair and flexible terms. We have also focused our efforts in project management, operations and fulfillment, positioning us to respond quickly and effectively manage your custom projects. Expansion of our quality, regulatory affairs and quality control infrastructure underscores our commitment to making the best possible products, every time.

Most importantly, we pride ourselves in being a resource to you, not just through our product offering, but through the support that we provide. As one of the few privately held molecular biology tools providers, our goal is to establish partnerships that advance our customer’s science and business objectives. 100% of our production capacity is earmarked for the customers we serve, and not for the manufacture of our own products.

Amplification-based Applications

NEB has a long history in the development of reliable and convenient tools for amplification, and offers a large selection of products for PCR, qPCR, RT-qPCR and isothermal amplification. Our extensive expertise in this area has allowed us to develop optimized enzymes for a variety of applications, including incorporation into customers' diagnostics. Bulk and/or custom formats are available for all products, with more specific customization details included below:

Application Products Product Notes Custom Formulations Available
PCR Applications qPCR/RT-qPCR

DNA, Dye

  • Luna® Universal qPCR Master Mix

DNA, Probe

  • Luna Universal Probe qPCR Master Mix
  • Compatible with automation and reaction miniaturization
  • Room temperature stable for ≥ 24 hours
  • Blue-dye-free
  • Lyo-compatible

RNA (1-step), Dye

  • Luna Universal One-Step RT-qPCR Kit

RNA (1-step), Probe

  • Luna® Probe One-Step RT-qPCR 4X Mix with UDG
  • Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)
  • Luna® Universal Probe One-Step RT-qPCR Kit
  • Luna® Probe One-Step RT-qPCR Kit (No ROX)
  • Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit
  • LyoPrime Luna®Probe One-Step RT-qPCR Mix with UDG
  • Luna WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness
  • Compatible with automation and reaction miniaturization
  • Room temperature stable for ≥ 24 hours
  • Lyophilized format (NEB #L4001)
  • Blue-dye-free
  • Lyo-compatible
  • Add primers and probes (NEB #L4001)

RNA (2-step)

  • LunaScript® RT SuperMix Kit
  • Novel thermostable RT
  • Single-tube format
  • 13-minute DNA synthesis protocol
  • Blue-dye-free
PCR/RT-PCR

Master Mixes

  • Q5® Hot Start High-Fidelity 2X Master Mix
  • Q5 High-Fidelity 2X Master Mix

Standalone Enzyme & Buffer

  • Q5 Hot Start High-Fidelity DNA Polymerase
  • Q5 High-Fidelity DNA Polymerase
  • ~280X fidelity of Taq
  • Consistent, fast, reliable performance
  • Compatible with automation and reaction miniaturization
  • Room temperature stable for ≥ 24 hours
  • High conc.
  • Glycerol-free
  • Custom mixes
Z05 DNA Polymerase
  • RNA- and DNA- dependent DNA polymerase activities in a single enzyme
  • +/- Hot start
Q5 Blood Direct 2X Master Mix
  • Amplification direct from blood

Hot Start Taq DNA Polymerase

Hot Start Taq 2X Master Mix
  • Unique aptamer-based enzyme control supports fast protocols
  • High conc.
  • Glycerol-free
Isothermal Applications LAMP/RT-LAMP

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

WarmStart Colorimetric LAMP 2X Master Mix with UDG
  • Fast, clear pink-to-yellow visible detection of amplification
  • Results in approximately 30 minutes
  • Automation - compatible when coupled with absorbance plate reader
SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit
  • Simple, colorimetric detection of amplification of SARS-CoV-2 nucleic acid

WarmStart LAMP Kit (DNA & RNA)

WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG)

LyoPrime WarmStart Fluorescent LAMP/RT LAMP Mix (with UDG)
  • Master mix for LAMP and RT-LAMP workflows
  • Supports multiple detection methods, including fluorescence and turbidity
  • Lyophilized format (NEB #L4401)
  • Lyo-compatible
  • High conc.
  • Add primers (NEB #L4401)
Tte UvrD Helicase
  • Improves specificity of LAMP reactions
  • High conc.

Bst-XT WarmStart™ DNA Polymerase

Bst-XT WarmStart DNA Polymerase (Glycerol-free)
  • Combines the high specificity of Bst 2.0 and the polymerization speed of Bst 3.0 DNA Polymerases
  • Broad optimal reaction performance from 50-70°C
  • Glycerol-free
  • High conc.

Bst 2.0 WarmStart DNA Polymerase

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free)

Bst 2.0 DNA Polymerase
  • Improved reaction properties compared to wild-type Bst DNA Polymerase
  • Increased dUTP tolerance enables carryover prevention
  • Glycerol-free
  • High conc.
Bst 3.0 DNA Polymerase
  • DNA binding domain fusion supports robust performance
  • Significantly increased RT activity up to 72°C enables single enzyme RT-LAMP
  • Glycerol-free
  • High conc.

WarmStart RTx Reverse Transcriptase

WarmStart RTx Reverse Transcriptase (Glycerol-free)
  • In-silico designed RT for RT-LAMP with reversibly-bound aptamer that inhibits activity below 40°C
  • Glycerol-free
  • High conc.
Rolling Circle Amplification / Whole Genome Amplification

phi29-XT RCA Kit

phi29-XT WGA Kit
  • Includes phi29-XT DNA Polymerase, an engineered polymerase that generates high product yield in a short time with improved thermostability and sensitivity
Strand Displacement

Nt.BstNBI

WarmStart® Nt.BstNBI

WarmStart® Afu Uracil-DNA Glycosylase (UDG)
  • High purity, high quality nicking endonuclease
  • WarmStart Afu UDG digest uracil-containing amplification products at temperature greater than 40°C
  • Glycerol-free
  • High conc.
Recombinant Polymerase Amplification

Bsu DNA Polymerase, Large Fragment

T4 Gene 32 Protein
  • Core components of RPA reaction
  • Can increase yield and efficiency of amplification reactions
  • Glycerol-free
Other Deoxynucleotide Deoxynucleotide (dNTP) Solution Mix
  • Custom conc.
Nuclease-free Water Nuclease-free Water
Antarctic Thermolabile UDG Antarctic Thermolabile UDG
  • Unique thermolabile version is completely inactivated in typical isothermal and RT-qPCR workflows
  • High conc.
Proteinase K Proteinase K
Thermolabile Proteinase K Thermolabile Proteinase K
  • Unique thermolabile version is completely inactivated in typical isothermal and RT-qPCR workflows
  • High conc.
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Next Generation Sequencing-based Molecular Diagnostics

Sequencing is enabling scientists to make rapid advances in epidemiology and surveillance, basic and clinical research, and diagnostics. A fast-growing number of methods are being developed to address whole genome, as well as targeted approaches. In all cases, streamlined workflows that result in high-quality, high yield libraries are critical towards optimizing your next generation sequencing (NGS) results. The table below summarizes some of the products available for scientists who are working to develop diagnostics. Bulk and/or custom formats are available for all products, with more specific customization details included below.
Application Products Notes
NGS-based Molecular Diagnostics DNA Sequencing DNA library preparation with enzymatic DNA fragmentation
  • NEBNext UltraExpress FS DNA Library Prep Kit
  • NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)
  • NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina®
  • NEBNext Ultra II FS DNA Module
  • NEBNext Ultrashear® FFPE DNA Library Prep Kit
  • NEBNext Ultra II Q5® Master Mix
  • NEBNext Adaptors and Primers
  • NEBNext UltraExpress™ DNA Library Prep Kit
  • Range of packaging options including 96-well plates
  • Component and kit customization
  • Kitting
  • Automation-compatible
  • Streamlined workflows
DNA library preparation for cfDNA or presheared DNA
  • NEBNext UltraExpress DNA Library Prep Kit
  • NEBNext Ultra II DNA Library Prep Kit for Illumina
  • NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®
  • NEBNext Ultra II End Repair/dA-Tailing Module
  • NEBNext Ultra II Ligation Module
  • NEBNext Ultra II Q5 Master Mix
  • NEBNext Adaptors and Primers
DNA Methylation Sequencing
  • NEBNext Enzymatic Methyl-seq Kit
  • NEBNext Enzymatic 5-hmC Kit
RNA Sequencing Depletion of abundant RNAs
  • NEBNext®Poly(A) mRNA Magnetic Isolation Module
  • NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat)
  • NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat)
  • NEBNext rRNA Depletion Kit (Bacteria)
  • NEBNext RNA Depletion Core Reagent Set for customized depletion
RNA library preparation
  • NEBNext™UltraExpress RNA Library Kit
  • NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina
  • NEBNext® Ultra II RNA First Strand Synthesis Module
  • NEBNext® Ultra II Directional RNA Second Strand Synthesis Module
  • NEBNext® Ultra II End Repair/dA-Tailing Module
  • NEBNext® Ultra II Ligation Module
  • NEBNext® Ultra II Q5 Master Mix
  • NEBNext® Adaptors and Primers
  • NEBNext® Immune Sequencing Kit (Human)
Virus sequencing and detection
  • NEBNext® ARCTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)
  • NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)
  • NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)
  • NEBNext® ARTIC SARS-CoV-2 RT-PCR Module
  • Q5 Hot Start High-Fidelity 2X Master Mix
  • NEBNext Ultra II End Repair/dA-Tailing Module
  • Blunt/TA Ligase Master Mix
  • NEBNext Quick Ligation Module
  • NEBNext Ultra II RNA Library Prep Kit for Illumina
  • NEBNext Adaptors and Primers

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