Glycobiology

Product Overview​

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

Publications related to Glycobiology
  1. Hagen, B.M., Sanders, K.M. (2006) Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am J Physiol Cell Physiol; 1, 750-6. PubMedID: 16738006
  2. O’Reilly, M.K., Zhang, G., Imperiali, B. (2006) In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry; 8, 9593-603. PubMedID: 16878994
  3. Sielaff I. et al. (2006) Protein function microarrays based on self-immobilizing and self-labeling fusion proteins Chembiochem; 7, 194-202. PubMedID: 16342318
  4. Eichler, R., Lenz, O., Garten, W., Strecker, T. (2006) The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol J; 3, 41. PubMedID: 16737539
  5. Macnaughtan, M.A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., Moremen, K.W., Prestegard, J.H. (2008) 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I J Am Chem Soc; 130, 11864-5. PubMedID: 18700760
  6. Zhuo, Y., Chammas, R., Bellis, S.L. (2008) Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis J Biol Chem; 283, 22177-85. PubMedID: 18676377
  7. Fujita, N., Tamura, A., Higashidani, A., Tonozuka, T., Freeze, H.H., Nishikawa, A. (2008) The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells FEBS J; 275, 788-98. PubMedID: 18215164
  8. Graham, D.R., Mitsak, M.J., Elliott, S.T., Chen, D., Whelan, S.A., Hart, G.W., Van Eyk, J.E. (2008) Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses Proteomics; 872, 23-37. PubMedID: 19072736
  9. Banala J. et al. (2008) Caged substrates for protein labeling and immobilization Chembiochem; 4, 38-41. PubMedID: 18033718
  10. Oh, D.B., Park, J.S., Kim, M.W., Cheon, S.A., Kim, E.J., Moon, H.Y., Kwon, O., Rhee, S.K., Kang, H.A. (2008) Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly Biotechnol J; 3, 659-68. PubMedID: 18320567
  11. Baudino L, Shinohara Y, Nimmerjahn F, Furukawa J, Nakata M, Martínez-Soria E, Petry F, Ravetch JV, Nishimura S, Izui S. (2008) Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions J Immunol; 181(9), 6664-9. PubMedID: 18941257
  12. Park, Y., Zhang, Z., Laremore, T.N., Li, B., Sim, J.S., Im, A.R., Ahn, M.Y., Kim, Y.S., Linhardt, R.J. (2008) Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica) Glycoconj J; 25, 863-77. PubMedID: 18670878
  13. Wagner-Rousset, E., Bednarczyk, A., Bussat, M.C., Colas, O., Corvaïa, N., Schaeffer, C., Van Dorsselaer, A., Beck, A. (2008) The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies J Chromatogr B Analyt Technol Biomed Life Sci; 872, 23-37. PubMedID: 18672411
  14. Piro, J.R., Harris, B.T., Nishina, K., Soto, C., Morales, R., Rees, J.R., Supattapone, S. (2009) Prion protein glycosylation is not required for strain-specific neurotropism J Virol; 83, 5321-8.
  15. Velho, A.M., Jarvis, S.M. (2009) Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp Cell Res; 315, 2312-21. PubMedID: 19379732
  16. Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. (2009) Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjugate Chem; 20, 1228-36. PubMedID: 19425533
  17. Lee, K.J., Jung, J.H., Lee, J.M., So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380, 223-9. PubMedID: 19167352
  18. Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. (2009) A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology; 57, 287-94. PubMedID: 19500602
  19. Lee, K.J., Jung, J.H., Lee, J.M,, So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380, 223-9. PubMedID: 19167352
  20. Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. (2009) Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol Biol; 534, 213-23.
  21. Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. (2009) Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res; 337, 79-89. PubMedID: 19415334
  22. Teuton, J.R., Brandt, C.R. (2007) Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells J Virol; 81, 3731-9. PubMedID: 17229687
  23. Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. PubMedID: 27033327
  24. Song, Y., Choi, M.H., Park, J.N., Kim, M.W., Kim, E.J., Kang, H.A., Kim, J.Y. (2007) Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans Appl Environ Microbiol; 73, 4446-54. PubMedID: 17513593
  25. Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., Tiemeyer, M. (2007) Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo J Biol Chem; 282, 9127-42. PubMedID: 17264077
  26. O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris (2017) Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex J Proteome Res; 16, 4237-4243. PubMedID: 28953389
  27. Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M. (2002) Modulation of postendocytic sorting of G protein-coupled receptors Science; 297, 615-20. PubMedID: 12142540
  28. Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B. (2005) N-Glycans of Caenorhabditis elegans are specific to developmental stages J Biol Chem; 280, 26063-72. PubMedID: 15899899
  29. Srikrishna, G., Brive, L., Freeze, H.H. (2005) Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem Biophys Res Commun; 332, 1020-7. PubMedID: 15922305
  30. Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D. (2005) Analysis and function of prototype foamy virus envelope N glycosylation J Virol; 79, 7664-72. PubMedID: 15919919
  31. Kalidas, C., Joshi, L., Batt, C. (2001) Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng; 14, 201-7. PubMedID: 11342718
  32. Vainauskas, S., Kirk, C.H., Petralia, L., Guthrie, E.P., McLeod, E., Bielik, A., Luebbers, A., Foster, J.M., Hokke, C.H., Rudd, P.M., Shi, X., Taron, C.H. (2018) A novel broad specificity fucosidase capable of core a1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes Sci Rep. PubMedID: 29934601, DOI: 1038/s41598-018-27797-0
  33. Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ (2014) Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster PLoS Genet; 10(5), e1004349. PubMedID: 24785692, DOI: 10.1371/journal.pgen.1004349
  34. Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis J Biol Chem; 289(19), 13347-61. PubMedID: 24668812, DOI: 10.1074/jbc.M113.543710
  35. Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE (2014) Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris Appl Environ Microbiol; 80(9), 2746-53. PubMedID: 24561590, DOI: 10.1128/AEM.03914-13
  36. Möykkynen T, Coleman SK, Semenov A, Keinänen K (2014) The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4- isoxazolepropionic acid (AMPA) receptor desensitization J Biol Chem; 289(19), 13197-205. PubMedID: 24652293, DOI: 10.1074/jbc.M113.526301
  37. Stech M, Quast RB, Sachse R, Schulze C, Wüstenhagen DA, Kubick S (2014) A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells PLoS One; 9(5), e96635. PubMedID: 24804975, DOI: 10.1371/journal.pone.0096635
  38. Kwon HM, Lee KH, Han BW, Han MR, Kim DH, Kim DE (2014) An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells PLoS One; 9(5), e97574. PubMedID: 24835440, DOI: 10.1371/journal.pone.0097574
  39. Haller G, Li P, Esch C, Hsu S, Goate AM, Steinbach JH (2014) Functional characterization improves associations between rare non-synonymous variants in CHRNB4 and smoking behavior PLoS One; 9(5), e96753. PubMedID: 24804708, DOI: 10.1371/journal.pone.0096753
  40. Wright CR, Brown EL, Della-Gatta PA, Ward AC, Lynch GS, Russell AP (2014) G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle Front Physiol; 5, 170. PubMedID: 24822049, DOI: 10.3389/fphys.2014.00170
  41. Wicht O, Burkard C, de Haan CA, van Kuppeveld FJ, Rottier PJ, Bosch BJ (2014) Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell J Virol; 88(9), 4943-52. PubMedID: 24554652, DOI: 10.1128/JVI.03451-13
  42. Botto L, Cunati D, Coco S, Sesana S, Bulbarelli A, Biasini E, Colombo L, Negro A, Chiesa R, Masserini M, Palestini P (2014) Role of lipid rafts and GM1 in the segregation and processing of prion protein PLoS One; 9(5), e98344. PubMedID: 24859148, DOI: 10.1371/journal.pone.0098344
  43. Itahana Y, Han R, Barbier S, Lei Z, Rozen S, Itahana K (2014) The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense Oncogene. PubMedID: 24858040, DOI: 10.1038/onc.2014.119
  44. Arakel EC, Brandenburg S, Uchida K, Zhang H, Lin YW, Kohl T, Schrul B, Sulkin MS, Efimov IR, Nichols CG, Lehnart SE, Schwappach B (2014) Tuning the electrical properties of the heart by differential trafficking of KATP ion channel complexes J Cell Sci; 127(Pt 9), 2106-19. PubMedID: 24569881, DOI: 10.1242/jcs.141440
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Product Listing

New England Biolabs offers a variety of endo- and exoglycosidases for the modification and removal of glycans from glycoproteins in glycobiology research. We also offer heparin lyases, also known as heparinases, for the digestion of heparin and heparan sulfate.

Glycobiology

  • Boletopsis grisea Lectin (BGL)
  • N-Glycopeptide Binding Protein
  • O-Glycoprotease (IMPa)

Endoglycosidases

  • Endo D
  • Endo F2
  • Endo F3
  • Endo H
  • Endo Hf
  • Endo S
  • Endoglycoceramidase I (EGCase I)
  • Endoglycosidase Reaction Buffer Pack
  • Endo-β-Galactosidase
  • O-Glycosidase
  • O-Glycosidase & α2-3,6,8 Neuraminidase Bundle
  • PNGase A
  • PNGase F
  • PNGase F (Glycerol-free)
  • PNGase F (Glycerol-free), Recombinant
  • PNGase F, Recombinant
  • Protein Deglycosylation Mix II
  • Rapid™ PNGase F
  • Rapid™ PNGase F (non-reducing format)
  • Remove-iT® PNGase F

Exoglycosidases

  • Protein Deglycosylation Mix II
  • α1-2 Fucosidase
  • α1-2,3 Mannosidase
  • α1-2,3,6 Mannosidase
  • α1-2,4,6 Fucosidase O
  • α1-3,4 Fucosidase
  • α1-3,4,6 Galactosidase
  • α1-3,6 Galactosidase
  • α1-6 Mannosidase
  • α2-3 Neuraminidase S
  • α2-3,6,8 Neuraminidase
  • α2-3,6,8,9 Neuraminidase A
  • α-N-Acetylgalactosaminidase
  • β1-3 Galactosidase
  • β1-3,4 Galactosidase
  • β1-4 Galactosidase S
  • β-N-Acetylglucosaminidase S
  • β-N-Acetylhexosaminidasef

Heparinases

  • Bacteroides Heparinase I
  • Bacteroides Heparinase II
  • Bacteroides Heparinase III

Glycoprotein Substrates

  • Fetuin
  • Rapid PNGase F Antibody Standard
  • RNase B

Glycobiology Buffers & Diluents

  • Endoglycosidase Reaction Buffer Pack