Monarch® Spin Columns S3B and Tubes
Product Information
Monarch Spin Columns S3B and Tubes are engineered and uniquely optimized for high-capacity RNA cleanup and purification. The columns are designed to minimize buffer retention and reduce the risk of carryover contaminants, enabling efficient recovery of highly pure RNA.
Low elution volumes are achieved even at high RNA input levels, enabling recovery of concentrated RNA for multiple downstream applications. These spin columns are compatible with microcentrifuges, supporting benchtop workflows, without the need for additional equipment and instruments such as FPLC/HPLC.
Other Monarch columns for RNA Cleanup are also available for 10 µg (NEB #T2037), 50 µg (NEB #T2047) and 500 µg (NEB #T2057) binding capacities.
```| Properties | Details |
|---|---|
| Also included as a component in | Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060) |
| Product format | Spin columns and collection tubes for nucleic acid purification |
| Technology and purification format | Silica membrane-based purification technology |
| Provided as | 50 columns and 50 collection tubes |
| Suitable for | Purifying and cleaning up RNA from various sources including in vitro transcription (IVT) samples, IVT samples synthesized with modified nucleotides, enzymatic reactions and RNA extracted by other methods |
| Nucleic acid binding capacity | Up to 3 mg of RNA |
| Equipment compatibility | Centrifuge |
This product is related to the following categories:
RNA CleanupReagents Supplied
The following reagents are supplied with this product:
```| Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|
| Monarch® Spin Columns S3B and Tubes | T2067-1 | 25 | 1 x 50 columns | Not Applicable |
| Monarch® Spin Collection Tubes | T2118-1 | 25 | 1 x 50 tubes | Not Applicable |
Related Products
Companion Products
NEB #T2060
```FAQs & Troubleshooting
FAQs
Refer to the applicable product FAQ and technical documentation for compatibility information.
```Refer to the current product protocol and FAQ documentation for the recommended elution volume.
```Refer to the current product protocol and technical documentation for information regarding alternative elution buffers.
```Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it.
Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual.
Further information regarding NEB product quality can be found through the NEB quality information resources.
```Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product.
The following file naming structure is used to name these document files:
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot.
The following file naming structure is used to name these document files:
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed.
The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in.
However, this research should always be done in safe and ethical manner. Learn more about NEB ethics through the applicable company resources.
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