Genome Editing with CRISPR/Cas

CRISPR-Cas-based techniques enable precise gene editing workflows, enrichment approaches and in vitro detection assays with easy retargeting. A Cas ribonucleoprotein (RNP) complex activates when its guide RNA binds to a target sequence and its Cas-interacting domain binds to a specific protospacer-adjacent motif (PAM) in a nucleic acid substrate. Delivery formats may incorporate plasmid constructs, viral vectors, mRNA molecules, sgRNA molecules and Cas proteins.