Competent Cells for High Throughput Cloning
Product Overview
When performing high-throughput cloning, having the right competent cells in the right format helps optimize your workflow. Invitrogen MultiShot formats of chemically competent cells are designed for compatibility with multichannel pipettes and automated liquid handling robots to streamline the bacterial transformation process.
MultiShot chemically competent cells are available in three packaging formats: StripWell, FlexPlate, and standard 96-well PCR plates. These formats offer flexibility in the number of transformations that can be performed at once while maintaining the same cloning workflow.
Compare competent cell formats for high-throughput cloning
StripWell format
- Format description 12 capped 8-tube strips in lidded storage rack
- Best for Flexible number of transformation reactions from 1 to 96
- Volume of competent cells per tube/well 50 μL/tube
FlexPlate format
- Format description Foil-sealed 96-well break-away PCR plate that can be separated into 12 8-well segments
- Best for High throughput cloning with ability to use part of the plate (multiples of 8) in a PCR plate format
- Volume of competent cells per tube/well 20 μL/well
StripWell format
- Format description Full-skirted 96-well plate
- Best for Full-plate high throughput and automated cloning workflows
- Volume of competent cells per tube/well 20 μL/well
Heat-sealed plates for competent cell storage
Store your valuable competent cells with confidence. Our 96-well and FlexPlate-formatted plates are heat-sealed with a robust foil that stands up to ultra-low temperature storage at –80°C without peeling or delamination. Designed for flexibility and ease of use, the seal can be easily removed by hand or integrated into automated workflows using liquid handling systems—streamlining your process from storage to transformation.
Coli strains available as MultiShot competent cells
All Invitrogen MultiShot competent cells are supplied as a kit including SOC medium and control plasmid.
| E. coli strain | StripWell format | FlexPlate format | 96-well plate format |
|---|---|---|---|
| TOP10 | C409601 | C4081201 | C40005 (5 plates), A40007058 (1 plate) |
| DH5α T1R | C449601 | C4481201 | A40007055 |
| DH10B T1R | – | C6481201 | – |
| Mach1 T1R | C869601 | C8681201 | A40007050 |
| OmniMAX 2 T1R | – | C8581201 | – |
| Stbl3 | C739601 | C7381201 | A40007054 |
| BL21 (DE3) | – | A40007053 | A40007051 |
| BL21 Star (DE3) | C609601 | – | – |
| See all products in this format | StripWell competent cells | FlexPlate competent cells | – |
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Resources
Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.
Competent cells educational resources
Review rich educational content on transformation workflow, troubleshooting, strategies, and selection guides.
Cloning webinars
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Short articles and digital handbook for cloning applications
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Which Competent Cell Strain is Right for You?
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