PCR, qPCR & Amplification Technologies

Product Overview​

NEB offers a wide range of DNA polymerases and reverse transcriptases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification and PCR. We offer a broad variety of DNA and RNA polymerases, nucleotide mixes, kits, master mixes and more, including:

  • Luna® qPCR and RT-qPCR Reagents: for rapid, sensitive and precise detection of RNA, DNA and cDNA targets
  • Q5® High-Fidelity DNA Polymerase: for robust ultra high-fidelity PCR
  • OneTaq®: for robust routine PCR
  • Phusion™: for high-fidelity PCR 
 

For technical properties and considerations for choosing a DNA polymerase or reverse transcriptase, please refer to these resources:

  • DNA Polymerase Selection Chart
  • Reverse Transcriptase Selection Chart
PCR, qPCR & Amplification Technologies includes these subcategories:
Q5® High-Fidelity DNA Polymerase
OneTaq® DNA Polymerases
Taq DNA Polymerase
LongAmp® DNA Polymerases
Luna® qPCR & RT-qPCR
Other PCR Polymerase
Phusion™ High-Fidelity DNA Polymerase
Master Mix
DNA Manipulation
Isothermal Amplification & Strand Displacement
Nucleotide Solution
cDNA Synthesis & Reverse Transcriptases
RT-PCR
Reaction Buffers
FAQs for PCR, qPCR & Amplification Technologies
  • Polymerases and Amplification FAQs
Protocols for PCR, qPCR & Amplification Technologies
  • Luna® Universal qPCR Master Mix Protocol (NEB #M3003)
  • Luna® Universal One-Step RT-qPCR Kit Protocol (E3005)
  • Luna® Universal Probe One-Step RT-qPCR Kit Protocol (E3006)
  • Luna® Universal Probe qPCR Master Mix Protocol (M3004)
  • Bst-XT WarmStart™ Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) (NEB #M1712)
  • PCR using Q5®-XT Hot Start High-Fidelity 2X Master Mix (NEB #M2499)
  • Luna® Probe One-Step RT-qPCR Kit (No ROX) Protocol (NEB# E3007)
  • PCR Using Q5 Quick-Load™ High-Fidelity 2X Master Mix (NEB #M0578)
Application Notes for PCR, qPCR & Amplification Technologies
  • High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB Luna Reagents
  • Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes
Tools & Resources

Feature Articles

  • Understanding Variability in DNA Amplification Reactions
  • DNA Damage - the major cause of missing pieces from the DNA puzzle
  • Anatomy of a Polymerase - How Function and Structure are Related

    Read about the relationship between Polymerase structure and function when copying DNA.

Brochures

  • Isothermal Amplification Brochure
  • Luna Universal qPCR And RT-qPCR Brochure
  • PCR Reagents Brochure

Web Tools

  • NEB LAMP Primer Design Tool

Selection Tools

  • DNA Polymerase Selection Chart

Troubleshooting Guides

  • PCR Troubleshooting Guide
  • Taq PCR Kit Troubleshooting Guide
  • Luna® qPCR Troubleshooting Guide
  • Luna® One-Step RT-qPCR Troubleshooting Guide

Usage Guidelines

  • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
  • DNA Damage and PreCR
  • Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
  • Guidelines for PCR Optimization with Taq DNA Polymerase
  • Guidelines for PCR Optimization with Thermophilic DNA Polymerases
  • Optimization Tips for Luna® One-Step RT-qPCR
  • Optimization Tips for Luna® qPCR
  • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit

Posters

  • Molecular Diagnostics for Gastrointestinal Parasites and Impact on Intestinal Microbiota in Rural Agentinian Children (2015)
  • A Single-tube, Low Input Protocol for Long Read Sequencing (2019)
  • Utilizing Lyophilized LAMP Reagents in Rapid Molecular Assays
Publications related to PCR, qPCR & Amplification Technologies
  1. Poole, C.B., Sinha, A., Ettwiller, L., Apone, L., McKay, K., Panchapakesa, V., Lima, N.F., Ferreira, M.U., Wanji, S., Carlow, C.K.S (2019) In silico identification of novel biomarkers and development of new rapid diagnostic tests for the filarial parasites Mansonella perstans and Mansonella ozzardi Scientific Reports; 9-1, 10275. PubMedID: 31311985, DOI: 10.1038/s41598-019-46550-9
  2. Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234, DOI: 10.3389/fmolb.2019.00028
  3. Vladimir Potapov, Jennifer L. Ong. (2017) Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One; PubMedID: 28683110
PCR Selection Tool
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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Product Listing

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.