Protein Expression
Product Overview
Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once expressed, may be toxic to the host. Thus, no single solution exists for the successful production of all recombinant proteins, and a broad range of expression tools is needed to ensure the successful expression of your target protein.
NEB’s portfolio of protein expression products includes a wide variety of tools, included our new NEBExpress® portfolio, and is supported by access to scientists with over 40 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours. Protein Expression at NEB
Protein Expression includes these subcategories:
FAQs for Protein Expression
Protocols for Protein Expression
- Quick Start Protocol for NEBExpress® Cell-free E. coli Protein Synthesis System
- Protein Synthesis Reaction using PURExpress (E6800)
- NEBExpress MBP Fusion and Purification System Quick Start Protocol (NEB #E8201)
- Simplified Expression and Purification Protocol (E6901)
- Protein Expression using the K. lactis Protein Expression Kit - Simultaneous Expression of Multiple Proteins
Application Notes for Protein Expression
- Transformation of SHuffle® Competent Cell Strains
- E. coli Lemo21(DE3): A T7 RNA Polymerase-based protein overexpression platform for routine and difficult targets
- Co-expression of Multiple Proteins in Kluyveromyces lactis
- Protein Expression with T7 Express Strains
- Use of the PURExpress® Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Cell-free Expression and Rapid Analysis of Single-Chain Antibody Fragments
- From DNA Template to BLI Kinetics in a Day - Using Octet® VHH Biosensors
Tools & Resources
Feature Articles
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
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Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
Brochures
Selection Tools
Posters
- NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)
- Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)
- Discovering the Maltose Binding Protein epitope tag for expressed proteins (2018)
- An E. coli Cell Lysate Based System for In Vitro Protein Synthesis
Publications related to Protein Expression
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners.
The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Product Listing
New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress® products, resulting from our 50+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.
