Glycobiology & Proteomics
Product Overview
Not only are proteins a major structural component of living systems, they can also be effector molecules whose states determine downstream activities. Therefore, studying the protein complement within a cell can reveal the mechanisms behind many of the cell’s responses to its environment. Given the vast number of applications for protein analysis, several tools and methods for its study exist; determining the correct method for your application is paramount to success.
Protein Analysis & Tools includes these areas of focus:
Phage Display
Protein Standards
Protein Labeling
Protein Labeling (SNAP/CLIP)
Peptide Ligation
Protein Phosphatases & Kinases
Proteomics
Protein Digestion
Pull-down Assays
FAQs for Protein Analysis & Tools
- What is Ph.D.™ Phage Display?
- Which residues does Endoproteinase AspN cut?
- Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
- Is there a simple way to remove Trypsin after protein cleavage?
- How does one do a Trypsin in-gel digest?
- I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
- What is the Proteinase K activity in commonly used buffers?
Protocols for Protein Analysis & Tools
- Endo H/Endo Hf Protocol
Application Notes for Protein Analysis & Tools
- Ph.D.™ Peptide Display Cloning System
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Intein-Mediated Protein Ligation IPL and Labeling with the IMPACT™ Kit
- Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry
- Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix
- Using Glycosidases to Remove, Trim, or Modify Glycans on Therapeutic Proteins
Tools & Resources
Feature Articles
Combating Neglected Diseases - a genomic approach to identify potential drug targets
Parasitic Infections in Humans
Understand the common types of parasitic infections in humans and why they are still increasing in tropical regions.
Applications of the Ph.D. Phage Display Peptide Libraries
Posters
- Crystal Structure of the 8 bp-Specific Restriction Enzyme SwaI (2015)
- NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)
- Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)
- Discovering the Maltose Binding Protein epitope tag for expressed proteins (2018)
- Cell-Free Production of Antibody Fragments for High-Throughput Analyses
- Automated Protein Expression and Purification of NEBExpress® Cell-free E. coli Protein Synthesis Reactions using NEBExpress Ni-NTA Magnetic Beads
Publications related to Protein Analysis & Tools
Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z. (2019)
Characterization of the internal translation initiation region in monoclonal antibodies expressed in
Escherichia coli.
J Biol Chem; 294(48), 18046-18056.
PubMedID: 31604819,
DOI: 10.1074/jbc.RA119.011008
Ke, Na; Berkmen, Mehmet; Ren, Guoping; (2017)
A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo.
Nat Chem Biol; 13, 1022-1028.
PubMedID: 28628094,
DOI: 10.1038/nchembio.2409
Legal Information
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Featured Products
Trypsin-ultra™, Mass Spectrometry Grade (Recombinant)
Streptavidin Magnetic Beads
Product Listing
Protein Analysis & Tools
Blue Prestained Protein Standard, Broad Range (11-250 kDa)
Cold-Active TEV Protease
Color Prestained Protein Standard, Broad Range (10-250 kDa)
NEBExpress® Ni Spin Columns
NEBExpress® Ni-NTA Magnetic Beads
NEBExpress® Salt Active Nuclease
NEBExpress® Salt Active Nuclease, GMP Grade
NEBExpress® Ni Resin
Ph.D.™-7 Phage Display Peptide Library Kit v2
Ph.D.™-C7C Phage Display Peptide Library Kit v2
Ph.D™.-12 Phage Display Peptide Library Kit v2
Trypsin-ultra™, Mass Spectrometry Grade (Recombinant)
Unstained Protein Standard, Broad Range (10-200 kDa)
α-Lytic Protease
Phage Display
Amylose Magnetic Beads
Amylose Resin High Flow
M13KO7 Helper Phage
Ph.D.™ Peptide Display Cloning System
Ph.D.™-12 Phage Display Peptide Library
Ph.D.™-7 Phage Display Peptide Library Kit v2
Ph.D.™-C7C Phage Display Peptide Library Kit v2
Ph.D™.-12 Phage Display Peptide Library Kit v2
Protein A Magnetic Beads
Streptavidin Magnetic Beads
Protein Standards
Blue Prestained Protein Standard, Broad Range (11-250 kDa)
Color Prestained Protein Standard, Broad Range (10-250 kDa)
Unstained Protein Standard, Broad Range (10-200 kDa)
Protein Labeling
Anti-MBP Monoclonal Antibody
Anti-SNAP-tag® Antibody (Polyclonal)
BG-GLA-NHS
BG-Maleimide
BG-PEG-NH2
BL21(DE3) Competent E. coli
CLIP-Biotin
CLIP-Cell™ 505
CLIP-Cell™ TMR-Star
CLIP-Surface™ 488
CLIP-Surface™ 547
CLIP-Surface™ 647
IMPACT™ Kit
Lemo21(DE3) Competent E. coli
NiCo21(DE3) Competent E. coli
pCLIPf Vector
pSNAPf Vector
pSNAP-tag® (T7)-2 Vector
RNase B
SNAP-Biotin®
SNAP-Capture Magnetic Beads
SNAP-Cell® 505-Star
SNAP-Cell® Block
SNAP-Cell® Oregon Green®
SNAP-Cell® TMR-Star
SNAP-Cell® 430
SNAP-Cell® 647-SiR
SNAP-Surface® 488
SNAP-Surface® 549
SNAP-Surface® 594
SNAP-Surface® 649
SNAP-Surface® Alexa Fluor® 488
SNAP-Surface® Alexa Fluor® 546
SNAP-Surface® Alexa Fluor® 647
SNAP-Surface® Block
SNAP-tag® Purified Protein
T7 Express Competent E. coli (High Efficiency)
T7 Express lysY Competent E. coli (High Efficiency)
T7 Express lysY/Iq Competent E. coli (High Efficiency)
Protein Phosphatases & Kinases
Adenosine 5'-Triphosphate (ATP)
cAMP-dependent Protein Kinase (PKA), catalytic subunit
Casein Kinase II (CK2)
Lambda Protein Phosphatase (Lambda PP)
p-Nitrophenyl Phosphate (PNPP)
Sodium Orthovanadate (Vanadate)
Proteomics
Endo D
Endo F3
Endo H
Endo Hf
Endo S
Endoglycosidase Reaction Buffer Pack
Endoproteinase AspN
Endoproteinase GluC
Endoproteinase LysC
Enterokinase, light chain
Factor Xa Protease
Furin
IdeZ Protease (IgG-specific)
O-Glycoprotease (IMPa)
O-Glycosidase
O-Glycosidase & α2-3,6,8 Neuraminidase Bundle
PNGase A
PNGase F
PNGase F (Glycerol-free)
PNGase F (Glycerol-free), Recombinant
PNGase F, Recombinant
Protein Deglycosylation Mix II
Rapid™ PNGase F (non-reducing format)
Remove-iT® PNGase F
RNase B
TEV Protease
Trypsin-digested BSA MS Standard (CAM-modified)
Trypsin-ultra™, Mass Spectrometry Grade
α1-2,4,6 Fucosidase O
α1-3,4 Fucosidase
α1-3,4,6 Galactosidase
α2-3 Neuraminidase S
α2-3,6,8 Neuraminidase
α2-3,6,8,9 Neuraminidase A
α-Lytic Protease
β1-4 Galactosidase S
β-N-Acetylglucosaminidase S
Pull-down Assays
50 ml Magnetic Separation Rack
Amylose Magnetic Beads
NEBExpress® Ni Spin Columns
NEBExpress® Ni-NTA Magnetic Beads
NEBExpress® Ni Resin
Protein A Magnetic Beads
Protein G Magnetic Beads
SNAP-Capture Magnetic Beads
