NEBNext® Companion Module for Oxford Nanopore Technologies® Direct RNA Sequencing
Product Information
The NEBNext Companion Module for Oxford Nanopore Technologies Direct RNA Sequencing is a convenient solution that simplifies direct RNA library preparation. It includes the ONT-validated NEB ligation and reverse transcription reagents for use with the ONT SQK-RNA004 protocol (non-barcoded workflow), provided in volumes optimized for this workflow.
Included Reagents:
- Induro Reverse Transcriptase
- Induro RT Reaction Buffer
- Deoxynucleotide (dNTP) Solution Mix
- NEBNext Quick Ligation Reaction Buffer
- T4 DNA Ligase
- RNase Inhibitor, Murine
- Nuclease-free Water
All reagents are manufactured to stringent quality standards. Each lot is performance tested as a complete set through direct RNA library preparation and sequencing on an ONT device to support consistent results.
This module is used in conjunction with the ONT Direct RNA Sequencing Kit (SQK-RNA004/-XL). The appropriate device-specific SQK-RNA004 protocol from ONT should be followed for use of these reagents.
The NEBNext Companion Module contains the NEB reagents recommended in the SQK RNA004 workflow and is used with the Oxford Nanopore Technologies Direct RNA Sequencing Kit (SQK RNA004/-XL) to prepare non-barcoded direct RNA sequencing libraries from poly(A)-tailed or total RNA. In this workflow, the RT Adapter (RTA) is ligated to poly(A) RNA, followed by reverse transcription to generate a complementary cDNA strand that stabilizes the RNA and improves sequencing output. The resulting RNA–cDNA hybrid is then ligated to the RNA Ligation Adapter (RLA) for loading and sequencing on Oxford Nanopore Technologies instruments.
This product is related to the following categories:
Library Preparation for Oxford Nanopore Technologies Next Generation Sequencing Library PreparationProduct Information
The NEBNext Companion Module for Oxford Nanopore Technologies Direct RNA Sequencing is a convenient solution that simplifies direct RNA library preparation. It includes the ONT-validated NEB ligation and reverse transcription reagents for use with the ONT SQK-RNA004 protocol (non-barcoded workflow), provided in volumes optimized for this workflow.
Included Reagents:
- Induro Reverse Transcriptase
- Induro RT Reaction Buffer
- Deoxynucleotide (dNTP) Solution Mix
- NEBNext Quick Ligation Reaction Buffer
- T4 DNA Ligase
- RNase Inhibitor, Murine
- Nuclease-free Water
All reagents are manufactured to stringent quality standards. Each lot is performance tested as a complete set through direct RNA library preparation and sequencing on an ONT device to support consistent results.
This module is used in conjunction with the ONT Direct RNA Sequencing Kit (SQK-RNA004/-XL). The appropriate device-specific SQK-RNA004 protocol from ONT should be followed for use of these reagents.
The NEBNext Companion Module contains the NEB reagents recommended in the SQK RNA004 workflow and is used with the Oxford Nanopore Technologies Direct RNA Sequencing Kit (SQK RNA004/-XL) to prepare non-barcoded direct RNA sequencing libraries from poly(A)-tailed or total RNA. In this workflow, the RT Adapter (RTA) is ligated to poly(A) RNA, followed by reverse transcription to generate a complementary cDNA strand that stabilizes the RNA and improves sequencing output. The resulting RNA–cDNA hybrid is then ligated to the RNA Ligation Adapter (RLA) for loading and sequencing on Oxford Nanopore Technologies instruments.
This product is related to the following categories:
Library Preparation for Oxford Nanopore Technologies Next Generation Sequencing Library PreparationReagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| E3440S | Induro® Reverse Transcriptase | E3437AVIAL | -20 | 1 x 0.048 ml | 200,000 units/ml |
| Induro® RT Reaction Buffer | E3438AVIAL | -20 | 1 x 0.192 ml | 5 X | |
| Deoxynucleotide (dNTP) Solution Mix | E3439AVIAL | -20 | 1 x 0.048 ml | 10 mM | |
| NEBNext® Quick Ligation Reaction Buffer | E3441AVIAL | -20 | 1 x 0.264 ml | 5 X | |
| T4 DNA Ligase | E3442AVIAL | -20 | 1 x 0.108 ml | 2,000,000 units/ml | |
| RNase Inhibitor, Murine | E3443AVIAL | -20 | 1 x 0.024 ml | 40,000 units/ml | |
| Nuclease-free Water | E3444AVIAL | -20 | 1 x 1.1 ml | Not Applicable |
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Quality, Safety & Legal
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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
