DNA Gyrase (E. coli)
Product Information
DNA Gyrase (E. coli) is a Type II topoisomerase that catalyzes the introduction of negative supercoils in DNA in the presence of ATP. The Gyrase holoenzyme is a heterotetramer made up of 2 GyrA (97 kDa) subunits and 2 GyrB (90 kDa) subunits.
Product Source
An E. coli strain containing the cloned GyrA and GyrB genes.
- Other
- DNA Modifying Enzymes & Cloning Technologies
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| M7636S | -20 | ||||
| DNA Gyrase (E. coli) | M7636SVIAL | -20 | 1 x 0.02 ml | 30,000 units/ml | |
| Gyrase Reaction Buffer | B7636SVIAL | -20 | 1 x 1 ml | 5 X |
Properties & Usage
Materials Required but not Supplied
- Nuclease-free Water (NEB #B1500)
Unit Definition
One unit is defined as the amount of enzyme required to convert 0.5 µg relaxed pUC19 plasmid to the supercoiled form in 1X Gyrase Reaction Buffer incubated for 15 minutes at 37°C in a total reaction volume of 30 µl.
Reaction Conditions
1X Gyrase Reaction Buffer
Incubate at 37°C
Heat Inactivation
65°C for 20 minutes
Advantages and Features
Features
- Enhanced reaction buffer for improved activity
- Stable formulation for storage at -20°C
Related Products
Companion Products
- phi29-XT RCA Kit
- T4 DNA Ligase
- Topoisomerase I (E. coli)
- 9°N™ Reverse Gyrase
- Monarch® Spin Plasmid Miniprep Kit
Product Notes
- The reaction requires ATP and Mg2+ and is further stimulated by spermidine. The supplied Gyrase Reaction Buffer incorporates these essential components within an optimized formulation, thereby facilitating enhanced gyrase activity.
- Proprietary reaction buffer enhances gyrase activity.
References
- Gellert, M et al (1976). PNAS. 73, 3872-3876.
- Higgins, N.P. et al. (1978). PNAS. 75, 1773–1777.
Protocols, Manuals & Usage
Protocols for this product
FAQs & Troubleshooting
FAQs
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
- M7636S_L_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- M7636S_v1_10315651
- M7636S_v1_10335933
- M7636S_v1_10344055
- M7636S_v1_10349898
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
- DNA Gyrase (E. coli)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner
