Clinical field

NAO® Basket

For rapid and efficient DNA recovery

 

NAO® Basket is a semi-permeable system designed for releasing and concentrating human DNA from swab samples or other specimens during the extraction step.

Download Brochure

No sample transfer

Lysis in the NAO® Basket

NAO® Basket retains the lysis buffer during the lysis step of the forensic sample. Upon centrifugation on a high-speed centrifuge, valves in the bottom of the NAO® Basket open up and allow the sample lysate to pass through. The lysate containing the nucleic acids is efficiently recovered and collected in the provided collection tube, whereas the substrate remains in the NAO® Basket.

Crime scene

Improve DNA yield from challenging samples

The combination of NAO® Basket and FLOQSwabs® provides more than 90% DNA recovery of the reference sample collected by the swab, particularly critical for crime scene evidence testing. The 20 mm breaking point designed on the 4N6FLOQSwabs® is compatible with the NAO® Basket.

How to use NAO®Basket

1. Break the FLOQSwabs® at the breaking point into the NAO® Basket.
2. Add the lysis buffer and incubate as per extraction kit instructions.
3. Upon centrifugation, the sample lysate passes through the basket and is available for testing at the bottom of the tube.

Please refer to the PI for the complete details of the validated procedure.

Lyse & centrifuge in the basket

Innovative product design allows the passage of DNA from samples into the tube using a tight leak-proof design that can endure vortexing, centrifugations, and various storage conditions.

Multiple configurations

NAO® Basket are available in several formats as single pouches or bulk packages, both with 1,5 ml or 2 ml vials.

Compatibility

NAO® Basket is compatible with commercial Nucleic Extraction Platforms.

Forensic DNA grade

ISO 18385:2016 compliance minimizes the risk of detectable human nuclear DNA contamination in products used by the global forensic community.

Into the Line

This product line groups a variety of product codes, each optimized to address specific needs.


Check here the summary of these features and contact us to discover the product code that best suits your need-mix and available in your Country.

Collection Sites
  • FLOQSwabs®
  • CLASSIQSwabs™
  • Fabrics
  • Leather
  • Cigarette butts
  • Chewing gum
  • Sanitary pads
Fields of Application
  • Forensics
  • Genetics
Suggested uses table. Please refer to your GLP procedures to choose the most appropriate device. The use of this product in association with diagnostic kits or instrumentation should be internally validated by the user.
Download product focus

Scientific Mentions

Always faithful to our scientific approach, we are eager to hear the proof of the success of our efforts and commitment.

J Transl Med 2017 – Tessitore MV et al.

In addition, we developed the homemade protocol for DNA extraction as detailed below. Flocked swabs shafts were snapped at the molded breakpoint, inserted in the Nucleic Acid Optimizer NAO® basket (Copan), placed in microtubes and filled with 300 μL of saline solution, 300 μL of ATL buffer and 7 μL of Puregene Proteinase K 20 mg/mL (Qiagen).

FSI Genetics 2017 – Ambers A. et al.

Bloodstains and saliva stains collected with 4N6 FLOQSwabs® were extracted using Nucleic Acid Optimizer NAO® Baskets (Copan) and the QIAamp® DNA Investigator Kit. Modifications were made to the extraction protocol according to Copan’s recommendations. Modifications included performing sample lysis in NAO® Baskets under static conditions, with no vortexing or shaking during the first two incubation steps.

Journal of Forensic Sciences 2018 – Bruijns BB et al.

The manufacturer of the nylon flocked 4N6FLOQSwabs® advises the use of a Nucleic Acid Optimizer (NAO®) to increase the recovery of DNA from the swab. The NAO® is a semi-permeable basket that is applied in combination with high-speed centrifugation to collect the eluted DNA sample from the swab.

Explore resources
linked to this line

Brochures & Material

Brochure

NAO®Basket

Download Product Focus