Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit
Product Information
The Monarch Mag Cell-free DNA (cfDNA) Extraction Kit is a magnetic bead-based method for reproducible extraction of circulating cell-free DNA (ccfDNA) from biofluids such as plasma, urine and CSF. The kit can be used to isolate cell-free DNA (cfDNA) for use in discovery and detection workflows including circulating tumor DNA (ctDNA) profiling, cancer biomarker discovery, and oncology diagnostics research. This kit enables efficient extraction of typical cfDNA fragments (150-300 bp), and as low as 50 bp. Our scalable extraction process permits flexibility in sample volume and promotes efficient utilization of reagents. The standard protocol of this extraction kit is designed for 2 ml sample inputs and can easily be scaled to accommodate 1-4 ml sample input volumes. Supplementary protocols enable additional functionality and recovery. This kit is compatible with several different sample collection tubes including standard anticoagulant tubes (EDTA, sodium citrate etc.), as well as other commercially available preservative tubes. The silica-coated magnetic beads, combined with the optimized buffer chemistry, ensure maximum binding and recovery of cfDNA. When integrated with NEB’s sequencing and amplification applications, our cfDNA extraction solution enables comprehensive and streamlined workflows to generate high-quality data from challenging biofluid samples.
Need automation support or a script? Submit a request using the Monarch Automation Support & Script Request form. Automation scripts are currently available for select platforms.
| Properties | |
|---|---|
| Purification Format | Magnetic bead |
| Compatible Platform | Manual or automated |
| Available Sizes | 2 ml samples × 20 preps 2 ml samples × 100 preps Scalable for sample volumes of 1–4 ml |
| Intended Application | Cell-free DNA extraction from liquid samples |
| Sample Type Compatibility | Plasma and urine (evaluated); cerebrospinal fluid (CSF) (suitable) |
| Elution Volume | As low as 15 µl, depending on sample input volume |
| Compatible Downstream Applications | cfDNA library preparation and sequencing for mutation detection, detecting methylation signatures, and amplification methods including digital PCR |
This product is related to the following categories:
- Magnetic Bead-Based Nucleic Acid Purification,
- Nucleic Acid Purification
This product can be used in the following applications:
- Nucleic Acid Purification
Kit Components
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| T4070V | Monarch StabiLyse™ DNA/RNA Buffer | T2111-2 | 25 | 1 x 63 ml | Not Applicable |
| Monarch® Buffer BX | T2041-1 | 25 | 1 x 20 ml | Not Applicable | |
| Monarch® Buffer BY | T1121-11 | 25 | 1 x 8 ml | Not Applicable | |
| Monarch® Buffer WZ | T1115-2 | 25 | 1 x 12 ml | Not Applicable | |
| Nuclease-free Water | B1500-1 | 25 | 1 x 14 ml | Not Applicable | |
| Monarch® Mag Beads M2 | T4105-1 | 25 | 1 x 2.2 ml | Not Applicable | |
| Proteinase K, Molecular Biology Grade | P8200SVIAL | -20 | 1 x 1 ml | 20 mg/ml |
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
|---|---|---|---|---|---|
| T4070S | Monarch StabiLyse™ DNA/RNA Buffer | T2111-3 | 25 | 1 x 210 ml | Not Applicable |
| Monarch® Buffer BX | T2041-3 | 25 | 1 x 122 ml | Not Applicable | |
| Monarch® Buffer BY | T1121-33 | 25 | 1 x 36 ml | Not Applicable | |
| Monarch® Buffer WZ | T1115-4 | 25 | 1 x 50 ml | Not Applicable | |
| Nuclease-free Water | B1500-2 | 25 | 1 x 25 ml | Not Applicable | |
| Monarch® Mag Beads M2 | T4105-2 | 25 | 1 x 9 ml | Not Applicable | |
| Proteinase K, Molecular Biology Grade | P8200SVIAL | -20 | 4 x 1 ml | 20 mg/ml |
Related Products
Companion Products
- Monarch® Mag Beads M2
Protocols, Manuals & Usage
Protocols for this product
- Reagent & Buffer Preparation Guidance for Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)
- Protocol for Extraction of Cell-free DNA Using the Monarch® Mag Cell-Free DNA (cfDNA) Extraction Kit (NEB #T4070) in a Manual Workflow
- Protocol Guidance for Extraction of Cell-free DNA Using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070) in an Automated Instrument Workflow for the KingFisher Flex
- Protocol for Enhanced Recovery of Ultrashort (<100 bp) Cell-Free DNA Using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070) in a Manual Workflow
- Protocol for Additional Concentration of Cell-free DNA Post-Extraction Using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070) in a Manual Workflow
Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
- manualT4070
Usage Guidelines
- Important Procedure Notes when using the Monarch® Mag Cell-Free DNA (cfDNA) Extraction Kit (NEB #T4070)
FAQs & Troubleshooting
FAQs
- What sample types can I use with the Monarch Mag Cell-free DNA Extraction Kit (NEB #T4070)?
- Can I add Monarch StabiLyse DNA/RNA Buffer (NEB #T2111) to plasma and store it for extraction later?
- The Monarch Mag Beads M2 (NEB #T4105) have settled down at the bottom of the bottle. Is this a problem?
- Can I purchase the components of the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4105) separately?
- What is the composition of each reagent provided with the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)?
- When using the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070), instead of adding Monarch Buffer BX, Isopropanol and the beads separately into the sample, can I make a binding mix of these three components?
- What is the lowest elution volume I can use when using the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)?
- When using the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070), what concentration of the DNA needed for downstream applications?
- When using the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070), I noticed that wash buffer volumes are the same regardless of the sample input volume. Can I use lower volumes of wash buffers with low sample volume?
- I am interested in cfRNA extraction. Can I use the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070) for cfRNA extraction?
- I have used all the Proteinase K (NEB #P8200) included in the Monarch kit. Can I purchase more?
- Can I use NEB’s 50 ml Magnetic Separation Rack (NEB #S1507) with the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070) and Monarch Mag Cell-free RNA (cfRNA) Extraction Kit (NEB #T4080) for manual extractions? Are there recommended magnetic racks for cfDNA and cfRNA applications?
- How can I request a script and support for automation on my platform?
Troubleshooting
- Troubleshooting Guide for Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
- T4070S_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- T4070S_v1_10323140
- T4070S_v1_10325865
- T4070V_v1_10323143
- T4070V_v1_10334487
- T4070S_v1_10337087
- T4070V_v1_10337091
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
- Monarch StabiLyse™ DNA/RNA Buffer
- Monarch® Buffer BX
- Monarch® Buffer BY
- Monarch® Buffer WZ
- Nuclease-free Water
- Monarch® Mag Beads M2
- Proteinase K, Molecular Biology Grade
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner.
