Protein Concentrators
Protein concentrators are lab devices that use methods like centrifugal force or tangential flow filtration with a semi-permeable membrane to separate and concentrate proteins from a larger liquid volume by allowing smaller molecules to pass through while retaining larger protein molecules. Protein extraction, purification, and cleanup methods frequently produce dilute samples of proteins in buffer matrices incompatible with downstream applications. Thermo Fisher Scientific offers products that utilize two general methods of biomolecule concentration: ultrafiltration and crossflow filtration, also called tangential flow filtration (TFF).
Ultrafiltration centrifugal concentrator devices
Pierce Protein Concentrators are disposable ultrafiltration centrifugal devices used to concentrate, desalt, and buffer exchange biological samples, such as proteins and nucleic acids, readying them for downstream applications with minimal sample handling. They are available in four sizes (0.5, 6, 20, and 100 mL) and offer pH compatibility over a broad pH range (1–9).
Crossflow filtration concentrator devices
Nanocon Crossflow Concentrator is a concentrator that utilizes tangential flow centrifugation technology to concentrate nanosized particles including exosomes, lipid nanoparticles (LNP), and viruses. The 30 nM pore size PE membrane is excellent for nanosized particles, and it fits conveniently in a 250 mL centrifuge adapter.
Pierce Crossflow Filtration Cassettes are membrane-based devices designed for concentrating larger volume sizes and operate via peristaltic pump pressure. They are designed for use in small-volume manufacturing and pilot-scale tangential flow filtration of biological samples yielding high recovery of peptides, proteins, and virus/viral particles. They are used to concentrate large supernatant volumes (0.5–6 L). Additional accessories are available for use with the crossflow filtration cassettes.
features
Features of protein concentrators
Ultrafiltration centrifugal protein concentrators are designed to remove excess buffer and small molecules while retaining the target proteins, making them excellent for preparing samples for downstream applications. Features include:
Fast
concentrate samples up to 10- to 50-fold in 5–30 minutes (times may vary with different MWCOs)
Flexible
suitable for sample volumes ranging from 100 μL to 100 mL, with a wide range of MWCO offerings
Efficient
recovery of >90% of target sample while removing contaminants or exchange buffers
Convenient
clear markings, wide sample chamber, and removable filtrate chamber make handling simple and easy
Instrument compatible
can be used with standard centrifuges with either fixed-angle or swinging-bucket rotors
features
Features of Nanocon concentrators
Nanocon crossflow concentrators are a distinct choice for concentration of biomolecules with features that include:
Advanced technology
utilizes the latest innovations in tangential flow centrifugation to concentrate nanoparticles like viruses and exosomes
Excellent enrichment
engineered to help deliver consistent and reliable results while maintaining sample integrity
Enhanced recovery
optimizes yield through efficient separation processes that reduce sample loss and improve recovery rates
Versatile application
suitable for a wide range of biological fluids, including urine and cell culture media, well-suited for enriching large sample volumes
features
Features of crossflow filtration concentrators
Crossflow filtration cassette concentrators offer several key features that make them effective for protein purification workflows:
Scalable
concentrate sample volumes ranging from 0.5–6 L
Fast
many concentration and protein recovery protocols can be completed in 30 minutes or less
Versatile
concentration cassettes are available in five different MWCOs and are compatible with buffers up to pH 13
Efficient
recovery of >99% protein (with 25 mL buffer rinse)
Single-use assemblies for fill-finish processes
The fill-finish product family offers configurable, single-use assemblies from a standard component catalog for drug product final fill applications.
Compounding assemblies
Discover our selection of 2D and 3D bags designed for powders and liquids, with optional integrated filtration. These purpose-built bags and tubing maintain sterility and support maximum product recovery. Each bag is pressure tested with helium gas to help ensure product integrity.
Filtration assembly
Pre-Use Post-Sterilization Integrity Testing (PUPSIT) compliant double filtration assemblies to support final fill-finish. Available to support a variety of volumes across your workflow.
Filling assemblies
Built from high integrity components designed to minimize leak and contamination risk. Filling assemblies are comprised of surge bags, tubing, and filling needles that are aseptically delivered into the isolator through a beta bag.
Experience the fill-finish 3D tour
Explore this interactive tool to discover how our fill-finish solution can significantly enhance drug product recovery and provide exceptional lead times. Our systems are designed to be highly customizable and configurable to meet diverse requirements for optimal efficiency and quality in aseptic filling operations.
Featured fill-finish components
Labtainer Pro System
The Labtainer Pro is a single-use bioprocess container system designed for secure fluid handling and storage with minimal product retention. It integrates the BioTitan retention device for enhanced reliability and ease of use.
BioTitan Retention Device
The BioTitan Retention Device helps enhance single-use assemblies with a secure 360-degree seal, replacing traditional cable ties. With zero leaks reported as of 2021, the BioTitan device helps eliminate sharp edges and simplify setup for improve reliability and integrity in bioprocessing workflows.
Beta bag
vailable in a variety of bag sizes and transfer port diameters to help securely deliver your fill-finish assembly into the isolator without introducing contamination. Updated packaging is designed to prevent accidental damage and reduce waste.
Related products
Bioprocessing films
Bioprocessing films are critical in the bioprocessing workflow, providing sterile, durable containment from cell cultivation and media storage to fill finish. We offer high-quality, single-use films that help ensure product integrity, reduce contamination risk, and enhance efficiency.
Sterilizing Grade Filters
Integrate LifeASSURE sterilizing grade filters into your Thermo Scientific single-use assemblies—offering you the confidence of quality filtration performance, improved workflow integration, and reliable global supply.
Explore BioProcess Insights
resources
Additional bioprocessing resources
Bioprocessing Blogs
Explore our collection of information and insights to support your drug development journey.
Events and webinars
Join us to learn, evaluate, and connect with experienced professionals in the field.
Bioprocessing resources
Find product guides, tools, and reference materials to help plan and optimize your workflows.
Contact us
Connect with a Bioprocessing representative or request more info.
Order protein concentrators by volume
Recommended accessories for Pierce Crossflow Filtration Cassettes
Pierce Crossflow Sample and Diafiltration Reservoir, 500 mL › Pierce Crossflow Y-Connector › Pierce Crossflow Filtration Accessory Pack ›Resources
Resources
- Dialysis products
- Zeba desalting products
Learning resources
- Protein Biology Learning Center
- Protein Isolation and Purification Information
- Overview of dialysis, desalting, buffer exchange and protein concentration
Support resources
- Life Sciences Support Centers
- Protein Purification and Isolation Support Center
- Protein Dialysis, Desalting, and Concentration Support
