Qubit NGS Library Quantification Assay Kit
The Invitrogen™ Qubit™ NGS Library Quantification Assay is an easy-to-use fluorescence-based assay for precise molar quantification of sequencing-ready Illumina next-generation sequencing (NGS) libraries using the Qubit™ Flex Fluorometer or compatible fluorescence microplate readers.
Catalog Number
Q34250
Quantity
1 Ea.
Product Overview
The Invitrogen™ Qubit™ NGS Library Quantification Assay is an easy-to-use fluorescence-based assay for precise molar quantification of sequencing-ready Illumina next-generation sequencing (NGS) libraries using the Qubit™ Flex Fluorometer or compatible fluorescence microplate readers. The assay selectively quantifies P5/P7-adapted double-stranded DNA libraries prior to sequencing, helping ensure optimal cluster density and successful sequencing runs.
Accurate determination of library concentration is a critical step in NGS workflows, as the amount of DNA loaded onto a flow cell directly impacts cluster density, data quality, and sequencing efficiency. The Qubit NGS Library Quantification Assay provides a dilution-free, time-saving alternative to qPCR-based library quantification methods while delivering comparable precision.
features
Key features and benefits
The streamlined workflow reduces hands-on time and minimizes potential user error, enabling efficient library quantification for both genomic core facilities and individual laboratories. By delivering precise quantification of sequencing-ready libraries, the Qubit NGS Library Quantification Assay helps ensure optimal cluster density on flow cells, improving sequencing outcomes.
Selective molar quantification
Measures only dual-adapted, sequencing-ready libraries for improved accuracy
Dilution-free workflow
Eliminates error-prone serial dilution steps required for qPCR
Broad dynamic range
Quantifies libraries from 0.8–46 nM without additional dilution
Robust performance
Accurately quantifies target libraries even in the presence of contaminating nucleic acids
Flexible detection
Compatible with the Qubit Flex Fluorometer and fluorescence microplate readers
Accessible and cost-effective
No need for dedicated qPCR instrumentation
Notes
Designed for use with the Qubit Flex Fluorometer (Cat. No. Q33327) and fluorescence-based microplate readers. The assay is not currently available for use on Qubit 4 Fluorometers.
Optimized for use with Qubit Flex Pyrogen Free Assay Tube Strips (Cat. No. Q32893).
Requires the use of the Qubit Flex System Verification Assay Kit (Cat. No. Q33254) prior to the first assay run.
Specifications
Product Specifications
Detection Method
Fluorescence
Emission
515 nm
Excitation
490 nm
No. of Reactions
96 Reactions
Product Type
Fluorescence Assay
Quantity
1 Ea.
Format
Kit
Isolation Technology
Magnetic Bead
Sequencing Type
Next Generation Sequencing
Unit Size
Each
Figures
Linearity
Qubit Flex Quick Reference
